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  6. Oligo analysis HPLC column OligoSep Cartridge

Oligo analysis HPLC column OligoSep Cartridge

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NUC-99-3550 / ADS Biotec

last updated:Jul 31, 2025

ADSTEC Corporation Bio Department
ADSTEC Corporation Bio Department
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High-performance liquid chromatography (HPLC) column for oligonucleotide analysis

The OligoSep Cartridge manufactured by ADS Biotec (formerly Transgenomic) is a column for oligonucleotide analysis using ion-pair reverse phase HPLC. The packing material consists of non-porous beads made of alkylated polystyrene-divinylbenzene. Due to the non-porous nature of the beads, it allows for higher precision purification. It can be used not only for quality control of oligonucleotides but also for high-precision analysis of both single-stranded and double-stranded DNA. It is compatible with various types of high-performance liquid chromatography (HPLC) systems. By using buffers manufactured by ADS Biotec as the mobile phase, high-precision separation can be achieved. Additionally, by following the procedures outlined in the instruction manual to wash the column with 75% acetonitrile, the column can be reused multiple times.

    High Performance Liquid Chromatograph
オリゴ分析用HPLCカラム バナー.png

Oligo analysis HPLC column OligoSep Cartridge

オリゴ分析用HPLCカラム バナー.png
オリゴ分析用HPLCカラム バナー.png
  • Related Link - https://www.ads-tec.co.jp/bio/hplc-na/oligo-sep-co…

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basic information

4.6 x 50 mm

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Applications/Examples of results

Oligonucleotide analysis

Detailed information

  • NUC-99-3550_graph.png

    Analysis example using OligoSep Cartridge

  • Omaha

    ADS Biotec, Inc. (formerly Transgenomic, Inc.) has been manufacturing and selling column buffers for nucleic acid analysis for over 20 years.

  • NUC-99-3550_graph.png

    Column: NUC-99-3550 Mobile phase: TEAA Buffer A, Buffer B Flow rate: 0.9 mL/min Temperature: 80℃ Injection volume: 5μL By performing the analysis with a gradient of TEAA Buffer A and Buffer B, we were able to detect 20mers and 21mers, respectively.

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OligoSep Series HPLC Product Catalog

OligoSep Series HPLC Product Catalog

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HPLC column for nucleic acid analysis manufactured by ADS Biotec.

HPLC column for nucleic acid analysis manufactured by ADS Biotec.

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ADSTEC

ADSTEC Corporation Bio Department

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ADSTEC Corporation has been committed to customer-oriented principles since its establishment in 2002, focusing on two main businesses: the import and sale of image-related equipment, and the development and manufacturing of gene-related equipment. We take pride in having established a system that allows us to respond to our customers' needs in a detailed and speedy manner, expanding our business network both in Japan and overseas in this rapidly evolving field. In these two areas, we aspire to be a group of experts who can assist with projects, including customer proposals, and we are dedicated to enhancing our technical skills as well as investing in staff education to cultivate "human power." Moving forward, we will continue to assess the trends of the times and provide and develop products that truly match our customers' needs.

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