For the purification of micro-scale small amounts of streptavidin fusion proteins! Streptavidin PhyTip column.
Automatic purification of biotin-tagged proteins The Streptavidin PhyTip column filled with a medium that immobilizes streptavidin affinity ligands enables the automatic affinity purification of recombinant biotin-labeled proteins. One of the attractive applications of the Streptavidin PhyTip column is the biotinylated protein assay (immunoprecipitation). It is compatible with major liquid handlers for chip-based chromatography. The use of the PhyTip column requires pipetting operations at low speeds, so a dedicated electric pipetter, such as the 'ME System', or a liquid handler robot is necessary. It is compatible with a wide range of resins and custom packing is also available. Custom filling of the desired resin into the chip is possible. *For more details, please visit our website or feel free to contact us.
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basic information
Resin volume: Minimum 5ul to maximum 360ul Chip sizes used: 200ul, 300ul, 500ul, 1000ul QTY/Box: 96 *For more details, please visit our website or feel free to contact us.*
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Applications/Examples of results
Small-scale affinity protein purification, such as immunoprecipitation.
Detailed information
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Dual Flow Chromatography Technology Hydrophilic screens are fixed above and below the resin filled in the chip, and unlike conventional unidirectional chromatography, DFC allows for repeated suction and discharge, improving the binding affinity with the resin. With the realization of pipetting operations, reproducible data can be obtained using electric pipettors or liquid handlers. Additionally, pipetting at low speeds enables purification under mild conditions without losing the original activity of the proteins.
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Company information
Biotage Japan Co., Ltd. is a wholly-owned subsidiary of Biotage AB, which is headquartered in Uppsala, Sweden (BIOT: NASDAQ/OMX Stockholm). Biotage AB has its roots in several venture companies that originated from Uppsala University, a leading science and engineering university in the Nordic region. After undergoing international acquisitions and restructuring throughout the 2000s, it has recently achieved remarkable growth as a global company.