Ideal for ion exchange on a microscale! Ion exchange PhyTip columns compatible with HTS.
Ion exchange chromatography is used as an orthogonal chemistry to obtain high-purity final products, serving as a second polishing step after another purification process or affinity chromatography purification. Ion exchange PhyTip columns are chip-type columns that utilize Dual Flow Chromatography technology, making them suitable for efficient parallel purification of small amounts of protein samples. They are compatible with major liquid handlers for chip-type chromatography. The use of PhyTip columns requires pipetting operations at low speeds, necessitating dedicated electric pipettors like the 'ME System' or liquid handler robots. They can accommodate a wide variety of resins and custom packing is also available. Product Information (4 products): Strong Anion exchange Weak Anion exchange Strong Cation exchange Weak Cation exchange
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basic information
Resin volume: minimum 5ul to maximum 360ul Chip sizes used: 200ul, 300ul, 500ul, 1000ul QTY/Box: 96
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Applications/Examples of results
It is an ion exchange PhyTip suitable for sample processing on a microscale and compatible with HTS using 96-well plates.
Detailed information
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Dual Flow Chromatography Technology Hydrophilic screens are fixed above and below the resin filled in the chip, allowing for repeated suction and discharge, which improves the binding capacity with the resin, unlike conventional unidirectional chromatography. The realization of pipetting operations enables reproducible data to be obtained through the use of electric pipettors and liquid handlers. Additionally, pipetting at low speeds allows for purification under mild conditions without losing the original activity of the proteins.
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Company information
Biotage Japan Co., Ltd. is a wholly-owned subsidiary of Biotage AB, which is headquartered in Uppsala, Sweden (BIOT: NASDAQ/OMX Stockholm). Biotage AB has its roots in several venture companies that originated from Uppsala University, a leading science and engineering university in the Nordic region. After undergoing international acquisitions and restructuring throughout the 2000s, it has recently achieved remarkable growth as a global company.