What is the difference between isocratic elution and gradient elution? Presentation of explanatory materials available.
Understand how to choose between isocratic elution and gradient elution! A thorough and easy-to-understand explanation of their features, differences, and suitable scenarios! Free basic knowledge materials available!
In HPLC, there are isocratic elution, which maintains the composition of the mobile phase for separation, and gradient elution, which varies the composition of the mobile phase for separation. Generally, isocratic elution is used for analyzing multiple components where there is little difference in retention time for each component, while gradient elution is used for analyzing multiple components with significant differences in retention time. This document provides a clear summary of the fundamentals of separation in liquid chromatography (LC), detectors, and principles. It is recommended for those who are new to learning HPLC, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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【Other Published Content】 ■Peak Identification and Quantification in HPLC ■Terminology in Chromatography ■History of Agilent LC ■Agilent InfinityLab LC System/Solution Portfolio *For more details, please download the PDF or feel free to contact us.
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