A thorough explanation of one of the UV-visible absorbance detectors, DAD. We will also clearly explain the differences with VWD! Basic knowledge materials are available for free.
DAD, unlike VWD, irradiates the cell with light from the light source without spectrally analyzing it. The light that passes through the cell is spectrally analyzed and detected by a device with an array of photodiodes (photodiode array). It is possible to record the UV-Vis spectrum of the eluent from the column. Therefore, simultaneous multi-wavelength detection, peak purity testing, and comparison with the UV-Vis spectrum of standard substances can be performed, making the peak identification capability superior to that of VWD. This document provides a clear summary of the fundamentals of separation in liquid chromatography (LC), detectors, and principles. It is recommended for those who are newly learning HPLC, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.
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【Other Published Content】 ■Peak Identification and Quantification in HPLC ■Terminology in Chromatography ■History of Agilent LC ■Agilent InfinityLab LC System/Solution Portfolio *For more details, please download the PDF or feel free to contact us.
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Agilent was born out of the Hewlett-Packard Company's spin-off in 1999. Since then, we have received numerous awards related to innovation and have obtained patents for inventions that surpass those accolades. More importantly, the number of customers we serve has been increasing year by year, demonstrating the strength of our partnerships. Agilent has become a leader in the life sciences, diagnostics, and applied chemistry markets. We provide instruments, services, consumables, applications, and expertise to laboratories around the world, contributing to the discovery of insights that our customers are exploring. Through our expertise and reliable collaboration, we offer highly dependable solutions to our customers.