Quality control of choline in health supplements using HPLC-CAD.
[Introduction of Method Development Case] High-Precision Quality Control and Impurity Analysis Using HPLC Charged Aerosol Detector (CAD) Without the Need for Derivatization Method.
The HPLC-CAD system analyzes choline and its counterions, as well as impurities of choline, with high precision without the need for derivatization. The analysis conditions using mixed-mode with hydrophilic interaction chromatography (HILIC) accommodate both charged particle detection and mass spectrometry, enabling the simultaneous analysis of anions and cations, which allows for rapid and reliable detection of choline content and impurities. In this application note, we successfully detected the content of choline derivatives such as choline chloride, choline bitartrate, and choline citrate, along with their impurities, with high precision. Choline chloride, which had poor responsiveness with conventional UV detectors, can also be directly detected without the use of derivatization methods. Furthermore, the mixed-mode using HILIC accurately identifies impurities while avoiding interference from counterions. This serves as a powerful tool for ensuring safety and efficacy in quality control for dietary supplements and other products.
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basic information
Features of the Thermo Scientific Vanquish CAD Charged Particle Detector - High sensitivity analysis with 100 times the RI and over 10 times the ELSD (ng) - Weight-dependent response that does not rely on the chemical structure of the analyte - Useful for detecting all non-volatile and semi-volatile substances that cannot be detected by UV detectors or are difficult to detect by mass spectrometry due to low ionization efficiency
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Applications/Examples of results
Useful application examples: - Sugar analysis: High sensitivity detection without derivatization - Semi-quantitative analysis of components for which standard samples are difficult to obtain, such as natural products and food ingredients - Simultaneous analysis of raw materials and counter ions - Impurity testing in organic synthesis or manufacturing processes - Simultaneous analysis of surfactants and additives For more details, please refer to the PDF or feel free to contact us.
Detailed information
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1. Spraying: The solution is sprayed to form droplets, which enter the drying tube. 2. Evaporation/Particle Formation: The droplets dry inside the drying tube and become particles. 3. Charging: Charged nitrogen gas transfers charge to the particles in proportion to their diameter. 4. Detection: The charge on the particles is discharged and measured with a voltmeter.
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Company information
Thermo Fisher Scientific K.K., the Japanese subsidiary of Thermo Fisher Scientific Incorporated, is a sales company for comprehensive system analysis instruments, bio-related equipment, and research reagents. In Japan, it has bases in Yokohama, Tokyo, Osaka, Fukuoka, and Nagoya, handling various analytical instruments including mass spectrometers, various bio-related equipment, measuring instruments, medical devices, lab software, and research reagents.
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