[Case Study] Impurity Analysis of Amino Acids Using HPLC-UV-CAD

Achieving cost reduction and highly reliable analysis: The combination of charged particle detectors (CAD) and UV detectors allows for the simultaneous analysis of amino acids and impurities without derivatization.

This application introduces a method for the highly sensitive and robust analysis of L-aspartic acid and glycine impurities using the HPLC-UV-CAD method. The conventional derivatization methods used in amino acid analysis have faced challenges such as human error and variability in derivatization efficiency; however, this method provides a more reliable approach by detecting without derivatization. Additionally, it allows for the simultaneous analysis of amino acids and their impurities (especially organic acids) in a single chromatography run, significantly reducing analysis time and contributing to cost savings. Furthermore, the selectivity and limit of quantification (LOQ) of the new analytical method are equivalent to established official methods, making it useful for amino acid quality control. 【Features】 ■ Fewer errors compared to derivatization methods, offering greater robustness and reliability ■ Simultaneous detection of amino acids and organic acids using a single method, reducing analysis time and costs ■ Highly sensitive detection of amino acids through the combination of CAD and UV ■ The selectivity and limit of quantification (LOQ) of the new analytical method are equivalent to official methods *For more details, please download the PDF or feel free to contact us.

Analytical Equipment and Devices