[Case Study] High-Precision Profiling Technology of Polysorbate 80

HPLC-CAD and single quadrupole mass spectrometry can detect inter-lot comparisons and quality issues of Polysorbate 80!

This technical document explains an HPLC method suitable for the fingerprinting of Polysorbate 80 and proposes a simple approach to detect variations between different suppliers, grades, and manufacturing lots, as well as to identify quality issues. The Thermo Scientific ISQ EM single quadrupole mass spectrometer, with a mass range up to m/z 2,000, allows for the easy identification of a wide range of key components of polysorbates from low to high molecular weights. Additionally, the consistent response of the CAD and the reverse gradient method enable semi-quantification without standard substances, allowing for a substantial understanding of the mass balance between components with varying degrees of esterification. Please take a look at the optimal HPLC-CAD-MS method for fingerprinting Polysorbate 80. *For more details, please download the PDF or feel free to contact us.*

Other measurement, recording and measuring instruments