Established a simple and rapid screening method (TTC method) for E. faecalis and E. faecium!
The "TTC solution" is a simple screening reagent that establishes a rapid screening method (TTC method) for E. faecalis and E. faecium using the reduction ability of 2,3,5-triphenyltetrazolium chloride (TTC). TTC is originally white, but in physiologically active tissues, it is reduced by dehydrogenase enzymes to form an insoluble red compound called TPF (1,3,5-triphenylformazan). However, no color change occurs in tissues that lack activity. Various enzymes are involved in the reduction of TTC to TPF, including succinate dehydrogenase and flavin enzymes from the electron transport chain. 【Usage Instructions】 1. Collect one colony of bacteria grown on sheep blood agar at 37°C for 24 hours using a sterile swab. 2. Add 1-2 drops of TTC solution to the sterile swab that collected the colony. 3. Let it sit at room temperature for 2 minutes. *For more details, please download the PDF or feel free to contact us.*
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【Judgment Method】 ■Positive: Color change to red (presumed E. faecalis) ■Negative: No change (presumed E. faecium) *For more details, please download the PDF or feel free to contact us.
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As we enter the 21st century, the development of science and technology, including information technology (IT) and biotechnology, is accelerating, presenting new possibilities for people's lives. On the other hand, issues such as environmental pollution caused by chemical substances and the emergence and resurgence of infectious diseases due to microorganisms have not been overcome even with the latest scientific technologies, and are instead becoming more serious and complex. In this situation, the importance of the work of researchers, engineers, and healthcare professionals tackling these problems that threaten people's health will continue to grow. We aim to contribute to these individuals through the provision of instruments and consumables in the fields of environmental analysis, measurement, and microbiological research. We will reflect on what we can do and what we can uniquely offer, striving to provide unique value.
