Acquire high-function DNA sequences that recognize molecules using electrophoresis.
Life (Saitama University Research Seed Collection 2025-25 p.125)
Keywords: DNA aptamer, capillary electrophoresis, aptamer selection method, cells, exosomes, proteins
I am trying to create a method that can freely provide molecular materials capable of recognizing (binding to) any type of target molecule. I believe this is possible with DNA aptamers, which are DNA sequences that can recognize low to high molecular weight substances, cells, and more. To achieve this, I have established a method using capillary electrophoresis (CE), a separation technique, to rapidly and easily obtain aptamer sequences. In practice, I have demonstrated that it is possible to isolate and acquire (select) only the DNA that strongly binds to targets from mixtures of various types of DNA sequences and targets such as proteins, animal cells, and bacterial cells using CE. Additionally, I have already succeeded in developing high-functionality sequences through machine learning analysis of the obtained sequences and in creating molecules that exhibit high pharmacological activity by linking multiple aptamers. I believe that if molecular recognition elements can be obtained freely in this way, it will enable a wide range of applications, including the development of nucleic acid-based drugs, diagnostic reagents, and separation materials.
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Shingo Saito, Professor Graduate School of Science and Technology, Department of Materials Science, Basic Materials Area 【Recent Research Themes】 ● Development of a method for identifying metalloproteins in biological samples using gel electrophoresis ● Development of fluorescent reagents that recognize glycan chains ● Development of a safe electrophoretic measurement method for radioactive elements in high-dose samples ● Development of electrophoresis methods to analyze the interactions between environmental macromolecules (humic substances) and heavy metal ions
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【Appeal Points to the Industry】 ● Acquired high-performance DNA aptamers that recognize proteins, cells, and exosomes using capillary electrophoresis (Patent No. 6781883) ● Rapid selection specifically for biological particles such as animal cells, bacterial cells, and exosomes (Chem. Commun., 52, 461 (2016); Analyst, 142, 4030 (2017)) ● Analyzed high-performance DNA aptamer sequences using machine learning (Chem. Eur. J., 27, 10058 (2021)) ● Capable of designing multi-point recognition aptamers (Featured in Nikkei Industrial Newspaper, Application No. 2018-10307) 【Examples of Practical Use, Applications, and Utilization】 ● Acquired DNA sequences that bind to microbial cells such as E. coli, Bacillus subtilis, and yeast ● Acquired DNA sequences that bind to non-small cell lung cancer cell lines ● Developed novel DNA aptamers that demonstrate strong pharmacological activity by linking multiple aptamers ● Developed a method for rapid discrimination of DNA aptamer sequences by machine learning of sequence patterns
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The Saitama University Open Innovation Center is a center that functions as a liaison office for industry-academia-government collaboration. It consists of three departments: the Industry-Academia-Government Collaboration Department, the Intellectual Property Department, and the Startup Support Department, each staffed with coordinators well-versed in various fields. The center's activities include solving technical challenges in companies, supporting the implementation of joint research, and conducting technology transfer aimed at introducing and utilizing Saitama University's intellectual property.