[Case Study] Determination of Insertion Sites for Animal Cultured Cells and Plant Cells
We have added a case study of random integration analysis using the RAISING method!
We would like to introduce a case study of our random integration analysis service using the RAIS method. We performed the RAIS method on T-DNA taglines inserted into Arabidopsis thaliana using the Agrobacterium method, as well as on mouse cultured cells with retrovirus vectors inserted. We confirmed the insertion site sequences using Sanger sequencing and mapped the decoded sequences obtained from next-generation sequencing analysis to the genome sequence. As a result, it was confirmed that this method is effective for determining insertion sites in animal cultured cells as well as in plant cells. 【Random Integration Analysis Service using the RAIS Method】 ■ Sanger Sequencing Plan ■ Next-Generation Sequencing (NGS) Plan * For more details, please refer to the PDF document or feel free to contact us. * For case studies, please refer to the URL below.
- Company:ファスマック
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