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[Case Study] Efficient Analysis of Sunscreen Ingredients Using UHPLC System

[Case Study] Efficient Analysis of Sunscreen Ingredients Using UHPLC System

Rapid and accurate analysis of multiple ultraviolet filter components using a high-precision, environmentally friendly UHPLC method!

This technical document introduces a method for efficiently analyzing ten types of ultraviolet filter components contained in five types of sunscreen using a UHPLC system under a single analytical condition. The results of the validation of the method's accuracy and precision showed that the peak area RSD was below 1.10%, and the retention time RSD was below 0.50%, indicating high precision. Additionally, by transitioning the existing analytical method to the UHPLC method, we reduced solvent consumption and waste generation, and replaced tetrahydrofuran (THF) used in the sample solution with a more environmentally friendly solvent, thereby reducing the environmental impact. 【Features】 ■ Efficient analysis of ten types of ultraviolet filter components under a single analytical condition ■ High precision with peak area RSD below 1.10% and retention time RSD below 0.50% ■ Reduction of solvent consumption and waste generation through the UHPLC method ■ Thorough evaluation of accuracy, linearity, and precision ■ Use of environmentally friendly solvents to reduce environmental impact *For more details, please download the PDF or feel free to contact us.

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  • Other measurement, recording and measuring instruments
  • UHPLC

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[HPLC Case Study] Automatic Derivatization of Amino Acids and Quantification of D-Amino Acids

[HPLC Case Study] Automatic Derivatization of Amino Acids and Quantification of D-Amino Acids

High-precision and high-sensitivity analysis of D/L amino acids using online derivatization with an autosampler.

This application note introduces amino acid analysis utilizing the automatic derivatization feature of the Thermo Scientific Vanquish Flex UHPLC system. Online chiral derivatization using OPA/NAC was employed to conduct quantitative analysis of D-aspartic acid (D-Asp), D-glutamic acid (D-Glu), and D-serine (D-Ser). By automating the process from derivatization to injection within the auto-sampler, we streamline complex sample preparation while achieving high reproducibility and quantitativeness. Furthermore, we demonstrate the quantification of D-amino acids present in fermented foods such as apple cider vinegar, grain vinegar, and whey, showing that sensitive and selective analysis is possible even in complex sample matrices. **Features** - Separation of D and L forms of eight amino acids including D-Asp, D-Glu, and D-Ser in under 15 minutes. - High reproducibility confirmed with retention time RSD ≦ 0.16% and area value RSD ≦ 0.22%. - Good linearity in the calibration range of 1–500 ng/mL (R² ≧ 0.9999). - Examples of D-amino acid quantification in fermented foods (apple cider vinegar, grain vinegar, whey) along with chromatograms are presented.

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  • Analytical Equipment and Devices
  • UHPLC

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