大関 List of Products and Services

The "Cell-Free Protein Synthesis Service" is a service that utilizes the high protein synthesis capability of the silkworm silk gland. The silk gland is an organ specialized in silk protein synthesis, where a large amount of protein is synthesized. In this service, we have achieved a high protein synthesis yield of over 70μg/mL by using extracts from the posterior silk gland. It is possible to synthesize proteins derived from mammals, such as humans, with a high probability of solubility. Because synthesis can be done quickly, we can deliver the target protein in a short period. 【Features】 ■ Capable of synthesizing proteins that are cytotoxic ■ This system does not contain added reducing agents ■ Can synthesize proteins with disulfide bonds ■ No glycan modifications, so no different glycans are added ■ Contains no components derived from mammals *For more details, please refer to the PDF document or feel free to contact us.

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Our koji mold expression system is an excellent expression system for the production of secretory proteins/enzymes. Recognized for its high safety due to its long history of use in the production of fermented foods, koji mold has been utilized for many years. At Ozeki Corporation's Research Institute, we have successfully established a high-expression protein system using koji mold as a result of our unique technology development, and we offer contract expression services for proteins. There are cases where proteins that would form inclusion bodies in E. coli can be produced. 【Features】 ○ High-expression promoter 　→ By using an improved promoter that utilizes cis-elements, we achieve high transcription efficiency. ○ High translation efficiency 　→ High translation efficiency is realized by using effective 5' UTR sequences. ○ High-purity secretory production 　→ Due to the characteristics of this expression system, the amount of contaminating proteins in the culture medium is extremely low, allowing for the high-purity production of the target protein. ○ Expression success rate of 84%. For more details, please contact us or download the catalog.

• Other contract services