Peace of mind and safety! Production of recombinant proteins using koji mold.
Our koji mold expression system is an excellent expression system for the production of secretory proteins/enzymes. Recognized for its high safety due to its long history of use in the production of fermented foods, koji mold has been utilized for many years. At Ozeki Corporation's Research Institute, we have successfully established a high-expression protein system using koji mold as a result of our unique technology development, and we offer contract expression services for proteins. There are cases where proteins that would form inclusion bodies in E. coli can be produced. 【Features】 ○ High-expression promoter → By using an improved promoter that utilizes cis-elements, we achieve high transcription efficiency. ○ High translation efficiency → High translation efficiency is realized by using effective 5' UTR sequences. ○ High-purity secretory production → Due to the characteristics of this expression system, the amount of contaminating proteins in the culture medium is extremely low, allowing for the high-purity production of the target protein. ○ Expression success rate of 84%. For more details, please contact us or download the catalog.
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basic information
【Service Contents】 [Flow of Contracted Services] ○ Request ○ Information Disclosure (Non-Disclosure Agreement if necessary) ○ Quotation ○ Provision of Sample Genes ○ Construction of Expression Vectors ○ Transformation of Aspergillus ○ Purification of Transformants ○ Confirmation of Expression ○ Cultivation of Transformants [Options] ○ Artificial Gene Synthesis (Codon Optimization) ○ Self-Cloning ○ Scale-Up Cultivation ● For more details, please contact us or download the catalog.
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At Ozeki Co., Ltd. Research Institute, we continue to conduct research and development in the biotechnology of koji mold based on the know-how cultivated over many years in sake brewing. We have successfully developed a unique protein expression system and offer a "koji mold protein contract expression service" that maximally utilizes the characteristics of koji mold. The koji mold expression system can express complex structured proteins as an eukaryotic expression system, making it possible to mass-produce proteins that are difficult to produce in E. coli or yeast. We accommodate a wide range of protein expressions, from microbial to mammalian-derived proteins. We invite you to try our system.
