リプロセル List of Products and Services

PermeaPad Plate uses a unique lamination technology to pack a phospholipid membrane with a cellulose film membrane, creating an artificial membrane (PermeaPad). PermeaPad is used in artificial membrane permeability tests for early-stage drug screening. It can be stored at room temperature and does not require any pre-treatment, making it easy to handle. Due to its three-layer structure of <cellulose>-<phospholipid>-<cellulose>, PermeaPad is resistant to solvents and surfactants, allowing for permeability testing. Furthermore, it maintains stable permeability across a wide pH range (pH 1 to 10), making it less susceptible to pH gradients and enabling tests under various conditions. The PermeaPad Plate is in a 96-well plate format. The drugs added to the insert plate (or bottom plate) permeate to the bottom plate (or insert plate), and the concentration changes are analyzed using HPLC or LC-MS. The bottom plate can be set in a standard microplate reader, providing excellent versatility.

• Other Analysis

The compact design allows for multiple units to be installed within an incubator without taking up much space. Additionally, the absence of rust-prone screws makes cleaning and disinfection easy, resulting in low maintenance requirements. You can specify the shooting interval and duration to continuously capture images of the same plate inside the incubator. Images taken in time-lapse can be converted into videos, enabling the observation of cell growth and decline over time. Furthermore, it is possible to analyze cell counts and the occupied area of cells from the captured images. The control of the device is managed through dedicated software. The PC connects to the zenCELL owl via a wired connection (USB cable). The software allows for a list of images from all cameras, individual camera view settings, time-lapse, cell count, and calculation of the occupied area of cells.

• Other Analysis

Features - Manufacturing of mRNA/LNP for research and pharmaceutical applications - Encapsulation of mRNA using lipid nanoparticles (LNP) - mRNA with high expression efficiency of proteins - High-quality mRNA with low impurities (endotoxins, DNA, dsRNA, enzymes) Burnal Corporation has facilities capable of manufacturing mRNA and lipid nanoparticles (LNP) at GMP grade, allowing for the production of both research-grade and pharmaceutical-grade mRNA/LNP. By 2025, a new manufacturing facility will be operational, increasing the available quantity of mRNA from approximately 10 grams to around 100 grams, enabling supply at the level of approved drugs. This consistent provision of raw materials from early research to pharmaceuticals allows for uninterrupted development speed.

• DNA/RNA synthesis

Preci Inc. is a global vendor that provides high-quality and affordable human primary hepatocytes consistently. They have a track record of supplying approximately 8,000 vials of human primary hepatocytes annually, primarily to major pharmaceutical companies and CROs. Preci's human primary hepatocytes are suitable for various applications, including adherent culture, suspension culture, and spheroid culture. Particularly in products derived from multiple donors, they minimize variability in quality between batches, ensuring assay stability and reproducibility. Additionally, they provide not only frozen cells but also the media necessary for thawing and seeding.

• Human cells

"SNIPER" is a next-generation CRISPR/Cas9 technology suitable for SNP replacement. The reliability of providing target cells has significantly improved. It is possible to establish suitable genome editing conditions in a short period of time. We will propose appropriate editing designs and testing schemes according to your requests, so please feel free to consult with us. 【Features】 ■ Significantly improved reliability of providing target cells ■ Suitable genome editing conditions can be established in a short period of time ■ Early quantification of target cells is possible with our unique probes *For more details, please refer to the PDF materials or feel free to contact us.

• Other contract services

At Reprocell Co., Ltd., we offer efficacy evaluation tests using fresh, non-frozen human tissues tailored to your needs. It is also possible to utilize patient-derived tissues. Skin excised during surgery or skin obtained through biopsy can be cultured ex vivo for about 10 days. We provide photographs of schematic diagrams and HE-stained images of the cultured skin. Additionally, we include evaluations of inflammatory responses using skin and assessments of reactivity through local administration to the skin. 【Evaluating the original biological response with human skin tissue】 ■ Inflammatory response evaluation ■ Atopic dermatitis ■ Psoriasis ■ Ex vivo culture ■ HE staining ■ Cytokine measurement *For more details, please refer to the PDF materials or feel free to contact us.

• Other Testing Contract

At Reprocell Co., Ltd., we offer efficacy evaluation tests using fresh human tissues that are not frozen, tailored to your needs. It is also possible to utilize patient-derived tissues. We conduct ex vivo culture of the colon from IBD patients and perform measurements of cytokines, among other assessments. We provide images of response evaluations to drugs. Additionally, we have evaluations of inflammatory responses using skin and drug responsiveness using lungs. 【Efficacy Evaluation Using Fresh Human Tissues】 ■ Ulcerative Colitis ■ Psoriasis ■ Atopy ■ COPD ■ Crohn's Disease ■ Urinary Disorders ■ Asthma *For more details, please refer to the PDF materials or feel free to contact us.

• Corrosion evaluation test

We offer a service for establishing human iPS cells using the RNA method. Using reagents that can meet GMP grade standards, we deliver the fastest results in as little as 2 months. Additionally, we provide a "disease model cell creation service" that offers a one-stop solution from cell acquisition from disease patients to reprogramming and providing differentiated cells, so please take a look at that as well. 【Features】 ■ Safe ■ High quality ■ Delivered faster than anywhere else ■ Uses reagents that can meet GMP grade standards *For more details, please refer to the catalog or feel free to contact us.

• Human cells
• Cell-related contract
• Other contract services

The "StemFit AK02N" is a feeder-free culture medium for human iPS cells that minimizes variability due to manual techniques, allowing anyone to easily and stably increase cell numbers. It is possible to proliferate while maintaining undifferentiated characteristics at various cell densities. It achieves high cost performance in terms of the number of cells proliferated per cost, while also reducing the burden of medium exchange. 【Features】 ■ Minimal variability due to manual techniques ■ Ability to skip weekend medium exchanges ■ High cost performance achieved ■ Easy and stable cell proliferation for anyone ■ Proliferation while maintaining undifferentiated characteristics at various cell densities *For more details, please refer to the catalog or feel free to contact us.

• Other research reagents
• Other Culture
• Human cells

"NutriStem" is a maintenance medium for human ES/iPS cells that can be efficiently cultured from single cells. It can be used for both feeder-dependent and feeder-free cultures, and can be used as is without the need for supplement addition. Additionally, it is a product that can be widely used from the establishment to the maintenance culture of iPS cells. 【Features】 ■ Proven track record of differentiation induction ■ No need for supplement addition ■ Efficiently culturable from single cells ■ Usable for both feeder-dependent and feeder-free cultures *For more details, please refer to the catalog or feel free to contact us.

• Culture medium
• Other cells