マクロジェン・ジャパン List of Products and Services

We offer "knockout mice." Knockout (or gene targeting) technology is used to obtain organisms that exhibit new traits by disrupting specific genes, and it is very useful for understanding the in vivo functions of those genes. Please feel free to contact us when you need our services. 【Features】 ■ Very helpful for understanding the in vivo functions of specific genes ■ Uses 129sv strain ES cells ■ Donor is C57BL/6N strain *For more details, please refer to the related links or feel free to contact us.

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We offer "transgenic mice" at our company. With the completion of the human genome map, the functions of several genes have been clarified, but most genes remain unknown. Due to established experimental procedures and similarities to the human genome, TG mice are used for in vitro gene function research and as disease models, greatly contributing to advancements in the medical field. They are expected to become an important tool at the center of functional genomic research. [Production Process] ■ Construction of expression vector ■ Purification of expression vector ■ Microinjection ■ Identification of founder mice ■ Shipping *For more details, please refer to the related links or feel free to contact us.

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The "PacBio RSII/Sequel" is a sequencer that can obtain long reads. The long-read sequence data obtained not only facilitates the assembly of De novo whole genome sequencing but also contributes to structural analysis of genomes and identification of isoforms of transcripts. By utilizing our diverse range of short-read/long-read sequencers, we provide suitable solutions for whole genome analysis of various organisms. Please feel free to consult us regarding species for which whole genome analysis is challenging. 【Features】 ■ Adopts single-molecule real-time technology to achieve long reads ■ Capable of sequencing at the single-molecule level ■ Compatible with genomes of a wide range of GC content ■ Provides suitable solutions for whole genome analysis of diverse organisms *For more details, please refer to the related links or feel free to contact us.

• Other contract services

We would like to introduce our "Cancer Panel Sequence Analysis." Using the cancer panel, we can perform high-depth sequencing of all exons, including tumor suppressor genes and hotspot mutations. Analysis is possible from a variety of samples, including Formalin-Fixed Paraffin-Embedded (FFPE) DNA. Please feel free to contact us when you need our services. 【Platform】 ■ HiSeq / MiSeq system ■ Ion Torrent / Proton *For more details, please refer to the related links or feel free to contact us.

• Other contract services

The "HiSeqX" is a large-scale sequencer dedicated to whole genome sequence analysis that can provide high-throughput whole genome sequencing data at a low cost. In the new aligned flow cell, billions of nanowells are fixed in place, achieving uniform cluster spacing and uniform cluster sizes. The sequencing reads and the overall output of the system have significantly improved. By using this sequencer, we can offer whole genome sequence analysis at a reasonable price, supporting not only basic research but also its application in clinical research. 【Features】 ■ Capable of obtaining whole genome sequencing data for 1,800 individuals per year with a single unit ■ Significant improvement in sequencing reads and overall system output ■ Adoption of dedicated chemistry that contributes to high-throughput data ■ Providing whole genome sequence analysis at a reasonable price ■ Supporting applications in clinical research beyond basic research *For more details, please refer to the related links or feel free to contact us.

• Other contract services

The "NovaSeq6000" is a large-scale sequencer from Illumina that supports a wide range of applications, from whole genome sequencing to transcriptomics, by selecting the appropriate flow cell from multiple options. It can obtain a large amount of data in a single sequencing analysis, allowing for various applications to be provided quickly and at a low cost. To accommodate a wide range of customers, we offer a shared plan that allows for the analysis of multiple customers' samples together. 【Features】 ■ Supports a wide range of applications ■ Can obtain a large amount of data in a single sequencing analysis ■ Provides a wide range of applications quickly and at a low cost ■ Dramatically improves the amount of data per flow cell ■ Utilizes dedicated chemistry that contributes to high-throughput data *For more details, please refer to the related links or feel free to contact us.

• Other contract services

Our "Run Only Sequencing Service" allows customers to perform cycle PCR, purification, and dissolution into Hi-Di, while we only conduct the sequencing. Using the Applied Biosystems 3730xl DNA analyzer, we carry out DNA sequencing of up to 1200 bp. Additionally, we thoroughly manage customer information and analysis results through our own Laboratory Information Management System (LIMS). 【Features】 ■ Customers perform cycle PCR, purification, and dissolution into Hi-Di ■ We provide a service that only conducts the sequencing ■ Utilizes the Applied Biosystems 3730xl DNA analyzer ■ Conducts DNA sequencing of up to 1200 bp ■ Thorough management of customer information and analysis results through our own Laboratory Information Management System (LIMS) *For more details, please refer to the related links or feel free to contact us.

• Other contract services

Our "Pre-Mix Sequencing Service" performs DNA sequencing of up to 1200 bp using the Applied Biosystems 3730xl DNA analyzer. By providing samples mixed with primers and templates, you can utilize our sequencing service at a lower cost. Additionally, our proprietary Laboratory Information Management System (LIMS) thoroughly manages your information and analysis results. 【Features】 ■ Uses Applied Biosystems 3730xl DNA analyzer ■ Conducts DNA sequencing of up to 1200 bp ■ By providing samples mixed with primers and templates, you can utilize our sequencing service at a lower cost ■ Thorough management of your information and analysis results through our proprietary Laboratory Information Management System (LIMS) *For more details, please refer to the related links or feel free to contact us.

• Other contract services

Our "Genescan Service" offers genotyping, microsatellite analysis, and SNP analysis using the capillary sequencer ABI3730XL. We achieve low prices based solely on runs. Analysis is conducted at Macrogen in South Korea, and results are provided in FSA data files. Please feel free to contact us when you need our services. 【Features】 ■ Low prices based solely on runs ■ Provided in FSA data files ■ PCR products using fluorescently labeled primers are processed with ABI3730XL ■ Offers genotyping, microsatellite analysis, and SNP analysis *For more details, please refer to the related links or feel free to contact us.

• Other contract services
• Contract Analysis
• Analysis Services

MacroGen Japan Co., Ltd. handles "mice." The "knockout mouse" offers extensive experience and know-how in processes such as target vector design, ES cell culture, manipulator operation, and the breeding of SPF mice. Additionally, as an extra service, we provide mouse breeding, lineage maintenance, SPF environment creation and management, and embryo freezing under optimal conditions, all supported by a breeding system for SPF mice established through extensive experience and rigorous evaluation standards. 【Product Lineup】 ■ Transgenic Mice ■ Knockout Mice *For more details, please refer to the related links or feel free to contact us.

• Other contract services

MacroGen's oligonucleotide synthesis (primer synthesis) uses the Mermade 192E (Bioautomation, USA). Additionally, an automation system for the entire oligonucleotide synthesis (primer synthesis) process has been established using Janus and BiomekFX. We can synthesize a variety of oligonucleotides, including "custom oligos," "modified oligos," and "duplex oligos." 【Features】 ■ High-precision quality check (QC) ■ Provision of high-purity oligos at low cost ■ Diverse special oligonucleotide synthesis (primer synthesis) ■ Thorough management of contract information using an information management system *For more details, please refer to the related links or feel free to contact us.

• Other contract services

Our next-generation sequencing services combine established data analysis pipelines based on extensive experience with various sequencers such as HiSeq, NovaSeq, MiSeq, and PacBio, targeting a wide range of organisms from humans to microorganisms. They are utilized in diverse genomic research, including whole genome sequencing and transcriptome sequencing. Additionally, the MacroGen Group provides Total Genomic Solutions to researchers worldwide, leveraging accumulated technology and extensive experience, and is advancing cutting-edge genomic research utilizing next-generation sequencing. Please feel free to contact us if you have any inquiries. *For more details, please refer to the related links or feel free to contact us.*

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• Other contract services

The "PacBio RSII/Sequel system" is a next-generation sequencer that can obtain longer reads compared to previous technologies. The long-read sequence data obtained not only facilitates de novo whole genome sequencing assembly but also contributes to genome structural analysis and the identification of transcript isoforms. Due to the adoption of single-molecule real-time (SMRT) technology, it does not require PCR amplification, unlike conventional sequencers, allowing it to accommodate a wide range of GC content in genomic DNA. 【Features】 ■ Capable of obtaining longer reads compared to previous technologies ■ Contributes to genome structural analysis and the identification of transcript isoforms ■ Utilizes single-molecule real-time (SMRT) technology ■ Accommodates a wide range of GC content in genomic DNA *For more details, please refer to the PDF document or feel free to contact us.

• Other measurement, recording and measuring instruments

The "NovaSeq System" is a large-scale sequencer from Illumina that supports a wide range of applications, from whole genome sequencing to transcriptomics, by selecting the appropriate flow cell from multiple options. It can obtain a large amount of data in a single sequencing analysis, allowing for a wide range of applications to be provided quickly and cost-effectively. Additionally, improved aligned flow cells that enable ultra-high-density cluster formation and high-performance cameras have dramatically increased the amount of data per flow cell. 【Features】 ■ Supports a wide range of applications ■ Obtains a large amount of data in a single sequencing analysis ■ Dramatically increases the amount of data per flow cell ■ Utilizes globally proven SBS chemistry *For more details, please refer to the PDF materials or feel free to contact us.

• Other measurement, recording and measuring instruments

The "HiSeqX System" is a large-scale sequencer dedicated to whole genome analysis that can provide high-throughput whole genome sequencing data at a low cost. With a single sequencer, it can obtain whole genome sequencing data for 1,800 individuals per year (30x coverage). By utilizing high-density flow cells, analysis is performed using HiSeqX-specific SBS chemistry. 【Features】 ■ Provides high-throughput whole genome sequencing data at a low cost ■ Can obtain whole genome sequencing data for 1,800 individuals per year with a single sequencer ■ Enables ultra-high-density cluster formation ■ Significantly improves sequencing reads and overall system output ■ Adopts Sequencing By Synthesis (SBS) chemistry *For more details, please refer to the PDF document or feel free to contact us.

• Other measurement, recording and measuring instruments

We offer comprehensive services for analyzing the sequences of 16S/ITS/28(26)S rRNA, which are widely used for identifying microbial species, including DNA extraction, PCR, sequencing, assembly, and BLAST. We perform analyses targeting bacteria, fungi, and yeast, from DNA extraction to BLAST. Results will be reported within 4-5 business days after the arrival of the strain. We will notify you via email with the Result Report, Contig Data, Blast Data, and Raw Data. 【Features】 ■ Targets bacteria, fungi, and yeast ■ Analysis from DNA extraction to BLAST ■ Results reported within 4-5 business days after strain arrival ■ Guarantees over 1,300 bp for bacterial 16S ■ Simple phylogenetic tree available as an option (free) *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis

We conduct "general sequencing analysis" using the Applied Biosystems 3730xl DNA analyzer. By performing a quality check through electrophoresis and conducting analysis after concentration adjustment, we will report the results based on the samples you send us. Additionally, for first-time requests only, we are offering three reactions for free to allow you to try our services. We encourage you to give it a try. 【Features】 ■ Utilizes the Applied Biosystems 3730xl DNA analyzer ■ Sequences up to 1,200 bp ■ Results reported within the shortest time of 24 hours, typically 36 hours, and a maximum of 4 business days (for large volumes) after sample arrival *For more details, please refer to the PDF document or feel free to contact us.

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We offer microarray analysis services using Illumina and Agilent chips. As an officially designated service provider by Illumina, we can provide accurate data certified by Illumina. Additionally, we also accept general analysis consultations based on bioinformatics. 【Types of Services】 ■ Illumina Microarray ■ Agilent Microarray ■ Affymetrix Microarray ■ Validation 　・ RealTime PCR (Expression) 　・ TaqMan SNP assay *For more details, please feel free to contact us.

• Contract Analysis