ニコダームリサーチ List of Products and Services

■Recommended for those with the following development challenges: Want to promote anti-wrinkle products that "prevent fine lines caused by dryness (cosmetics)"." Want results delivered as soon as possible. Want to obtain data while keeping costs down. Want to recruit subjects tailored to the objectives while adhering to the testing content of the guidelines. ■Features: - Conducted at our partner testing facility in China. - The climate is drier than in Japan, allowing for a longer suitable testing period. - The testing facility has a large capacity, enabling results to be delivered in about four months from preparation. - Approximately 100,000 subjects available. - Testing content (compliant with guidelines) and measurement equipment are implemented under the same conditions as in Japan. *Delivery times may vary depending on the availability of the testing facility, so please inquire through the contact form on our website: https://www.nikoderm.com/?utm_source=ndr&utm_medium=ipros&utm_campaign=ipros

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■Product Lineup Human Corneal Epithelial (SkinEthic HCE) Human Vaginal Mucosal Epithelial (SkinEthic HVE) Human Gingival Epithelial (SkinEthic HGE) Human Oral Epithelial (SkinEthic HOE) Human Epidermis (SkinEthic RHE) Pigmented Epidermis (SkinEthic RHPE) Human Epidermal Model Langerhans cells (SkinEthic RHE-LC) Human Full-Thickness Skin (T-skin) *We may also accept models not listed. Please consult us. ■Features - Handling of OECD Test Guideline Compliant Models - SkinEthic HCE → Included in Eye Irritation Test (OECD TG492, TG492B) - SkinEthic RHE → Included in Skin Primary Irritation Test (OECD TG439, TG431) - 3D skin tissue models from EPISKIN, a leading company in the development and manufacturing of three-dimensional cultured tissues in France.

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■Recommended for those with the following development challenges: Want to label products such as cosmetics as "Patch Tested" Want to conduct patch tests for sensitive skin Want to proceed while consulting on what safety data should be obtained ■Features - Various safety evaluations for humans can be contracted - Regular testing conducted ■Examples of tests Patch test, sensitive skin patch test, cumulative skin irritation test, RIPT (cumulative irritation and sensitization), phototoxicity and sensitization tests, comedogenicity, stinging *Please check the testing schedule on our website. https://bit.ly/4dTgnIp

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■Recommended for those with the following development challenges: Want to obtain evidence data on the effectiveness of cosmetics and quasi-drugs for the skin to enhance market competitiveness. Want to obtain evidence data on the effectiveness of beauty devices for the skin. ■Features: - Customized testing plans proposed for each case based on development and research purposes. - By partnering with testing institutions around the world, it is possible to obtain various evidence and visual data. - Evaluation for in vitro / ex vivo / humans, various contract testing available. ■Testing examples: Skin permeability (transdermal absorption), moisturizing and barrier, whitening, anti-wrinkle (guideline evaluation, custom evaluation), hair growth, relaxation, UV protection, antiperspirant/deodorant, long-term use skin condition measurement (stratum corneum diagnosis, etc.). *Other effectiveness evaluations not listed here are also available for contract testing. For details, please contact us through our website. https://www.nikoderm.com/%E6%9C%89%E7%94%A8%E6%80%A7%E8%A9%95%E4%BE%A1-1/?utm_source=ndr&utm_medium=ipros&utm_campaign=ipros

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■Recommended for those with the following development challenges: Want to compare multiple material candidates at once and find strengths that differentiate from competitors. Want to investigate if there are unknown strengths in existing raw materials. Want to shorten the lead time for raw material development. Want to develop food ingredients as cosmetic raw materials. ■Evaluation Features: - In a single test, the functional potential of the test sample can be understood for approximately 180 genes related to skin function. - By using a three-dimensional cultured epidermal model, testing is possible even for poorly water-soluble substances. - Based on the results of the microarray and the purpose of data acquisition, we will propose evaluation systems (in vitro/human evaluation) to confirm in the next step. ■Test Overview: The test sample is applied to a three-dimensional cultured epidermal model or epidermal cells, and after culturing, RNA is collected. Gene expression analysis is performed using a one-color method with a DNA chip.

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■Recommended for those with the following development challenges: - Want to comprehensively understand the functions of raw materials on the skin and elucidate their mechanisms of action. - Want to know what functional properties raw materials and ingredients exhibit on the skin. - Want to investigate if there are unknown strengths in existing raw materials. - Want to shorten the development lead time. ■Evaluation Features: - Shortens the lead time for raw material and ingredient development. - Enables mechanism analysis of skin functions (moisturizing, barrier function, antioxidant effects, etc.) through functional-level protein analysis. - Uses human-extracted skin, allowing customization of test conditions (age, gender, skin type, etc.). - Capable of conducting tests under conditions that are difficult for humans, such as applying strong stimuli. ■Test Overview: Using human-extracted skin, we capture the expression fluctuations of proteins due to the use of test products and simulate their mechanisms of action. This test will be conducted at an overseas partner testing facility. For inquiries, please feel free to contact us through the inquiry form on our website. https://www.nikoderm.com/?utm_source=ndr&utm_medium=ipros&utm_campaign=ipros

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■Recommended for those with the following development challenges: - Want to elucidate the mechanisms of action of cosmetic and pharmaceutical ingredients on the skin. - Want to evaluate the skin permeability of cosmetic and pharmaceutical ingredients and quantify their metabolites. - Want to quantify the temporal changes of IL-8 after drug administration. - Want to conduct experiments under conditions that impose difficult stimuli or burdens on humans. ■Evaluation Features: - By using extremely fresh excised skin, it is possible to analyze the effects of test substances on the skin and their mechanisms in a state almost identical to human skin (such as stratum corneum barrier function). - In addition to measuring the amount of permeation of ingredients, it is also possible to quantify substances generated in the skin, such as metabolites and inflammatory factors. - It is possible to apply electrical or light stimuli, as well as create conditions of irritated skin, which are difficult to implement on humans. - Ideal for evaluating the usefulness of raw materials and formulations of cosmetics and pharmaceuticals, as well as physical stimulation from beauty devices. ■Test Overview: Extremely fresh human excised skin is used within a few hours after excision. A semi-permeable membrane is attached to the tip of a microprobe through which perfusion fluid circulates, and it is inserted into the dermis. The dialysis fluid after the use of the test substance is collected and quantified.

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