HORIZO(ホライゾ) List of Products and Services

Fluorescence has become a major challenge in Raman spectroscopy. Timegate Instruments' patented Timegated Raman technology was developed to address this challenge. The phenomena of Raman scattering and fluorescence occur on different time scales. Raman scattering occurs on a very short sub-picosecond (less than one trillionth of a second) time scale, while the decay time of fluorescence is characterized by being very long. By using time-gate technology, it not only distinguishes between fluorescence and Raman signals but also provides temporal information about the Raman signals. Additionally, delayed noise components such as thermal radiation are ignored in Timegate Raman, which means they do not affect measurements. This allows for the analysis of structural changes in materials that undergo polymorphism at high temperatures (500-900°C), such as spodumene, while suppressing the effects of fluorescence. Timegate Raman spectroscopy, equipped with a real fluorescence removal function, makes the development of biopharmaceutical processes faster and more reliable. We deliver data and results that were previously unattainable into your hands. Reference: https://youtu.be/bsi29R-K4bo

• Raman Spectrophotometer
• Culture vessel
• Reaction Crystallizer

This catalog introduces the "CellMaker Series," which is researched and developed by Cellexus International. It includes information on operational methods and system configurations, as well as various controllers, enclosures, and bioreactor bags. Additionally, examples of implementation results using this system are also included, so please make use of this information when considering implementation. [Contents (partial)] ■ Main Features ■ System Configuration ■ Software ■ Various CellMaker Controllers ■ Various CellMaker Enclosures *For more details, please download the PDF or feel free to contact us.

• Other physicochemical equipment

We would like to introduce a case study by Proteon Pharmaceuticals, a Polish company, which evaluated the amplification efficiency of Salmonella enterica enteritidis bacteriophage using the CellMaker single-use bioreactor system. The optical density of the bacteria was measured every 30 minutes. The dynamics of the optical density (OD) were compared between the culture of the bacteria alone and the culture with the addition of phage. The test results demonstrated successful culture and high-efficiency amplification of Salmonella enterica enteritidis serotype. Furthermore, process optimization was conducted to improve phage yield, and this process is now adopted as part of the production process. [Overview] ■ Equipment used: CellMaker single-use bioreactor system ■ Experiment content: Production of bacteriophage in Salmonella enteritidis culture *For more details, please download the PDF or feel free to contact us.

• Other physicochemical equipment

Introduction to the amplification of phages using the CellMaker Regular 8L system. First, 5L of low-sodium LB broth was pumped into the CellMaker bioreactor bag, and Agrobacterium inoculum was added. The optical density (OD) was measured at 600nm, and the initial OD value was recorded as 0.05. The temperature was maintained at 25°C by a Peltier plate mounted on the back of the enclosure unit. The experiment was stopped the following morning, approximately 15 hours after infection by the phages, and the final concentration of the elution was 1.25x10^10 PFU/mL. [Summary] ■ Equipment used: CellMaker Regular 8L system ■ Experiment content: Amplification of phages ■ Results - Concentration in the bag at the time of infection: 10^5 PFU/mL - Final concentration of the elution: 1.25x10^10 PFU/mL *For more details, please download the PDF or feel free to contact us.

• Other physicochemical equipment

Cellexus streamlines cell culture and fermentation processes with its single-use airlift bioreactor "CellMaker." This airlift technology utilizes a unique stirring method that is simpler and more effective compared to traditional methods. It achieves cost reduction and time savings while maintaining high productivity. Cellexus also offers a rental service for the "CellMaker" bioreactor. By using this service, you can try out the system before purchasing and select equipment that is suitable for your process. Customers who utilize the rental service also have the option to purchase at a discounted price. This creates a mechanism that allows for the introduction of a bioreactor suitable for research while minimizing risk. Cellexus is a company specializing in biotechnology equipment, based in Scotland. For product-related videos, click here: https://youtu.be/bk9GkMzRIEc For more details, please contact contact@sub.horizo.co.jp.

• Other reaction equipment