花市電子顕微鏡技術研究所 List of Products and Services

We would like to introduce a case study of the analysis of "nervous tissue" conducted by our company. There are organs with unique structures such as myelin sheaths and synapses, and various images can be obtained depending on the location. [Analysis Overview] ■ Analysis Target: Nervous Tissue ■ Organs with unique structures such as myelin sheaths and synapses are present ■ Various images can be obtained depending on the location *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis

Here we introduce a case study of biological analysis conducted by our company. "Yeast" is difficult to fix and observe. There are various organelles inside, and the organs that can be observed may differ depending on the state of the yeast. 【Analysis Overview】 ■ Analysis Subject: Yeast ■ Yeast is difficult to fix and observe ■ There are various organelles inside, and depending on the state of the yeast, the organs that can be observed may differ *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis

We would like to introduce a case study of the analysis of "muscle fibers" conducted by our company. It can be seen that actin and myosin are neatly arranged around the Z band. 【Analysis Overview】 ■Analysis Subject: Muscle Fibers ■It is clear that actin and myosin are neatly arranged around the Z band. *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis

We would like to introduce a case study of the analysis of "Corneal Epithelium (Cross-section)" conducted by our company. By observing cross-sections with TEM, it is possible to examine everything from corneal epithelial cells to the endothelium. High magnification observation allows for the examination of cell adhesion states and more. 【Analysis Overview】 ■ Analysis Target: Corneal Epithelium (Cross-section) ■ Cross-section observation using TEM ■ Capable of observing from corneal epithelial cells to the endothelium ■ High magnification observation allows for examination of cell adhesion states and more *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis

We would like to introduce a case study of biological analysis conducted by our company. The analysis target is "corneal epithelium (SEM)." This is an overview image of corneal epithelial cells, allowing for a comparison of the microvilli of each individual cell. 【Analysis Overview】 ■ Analysis Target: Corneal Epithelium (SEM) ■ Overview of Corneal Epithelial Cells ■ Comparison of Microvilli of Each Individual Cell *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis

We would like to introduce a case study of biological analysis conducted by our company. The subject of analysis is "mold," which consists of mycelium and spores. Since the spores are easily detached, sample preparation requires caution. [Analysis Overview] ■ Subject of analysis: Mold ■ Composed of mycelium and spores ■ Sample preparation requires caution due to easily detached spores *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis

We would like to introduce a case study of the analysis of "triadenovirus" conducted by our company. Adenovirus is said to be a major pathogenic virus for colds and is a spherical particle with a diameter of approximately 80 nm. Using negative staining methods, the surface spikes can be observed. 【Analysis Overview】 ■ Analysis Subject: Triadenovirus ■ Adenovirus is a major pathogenic virus for colds ■ Spherical particle with a diameter of approximately 80 nm ■ Surface spikes can be observed using negative staining methods *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis

We would like to introduce a case study of the analysis of "pathogenic Escherichia coli O-157" conducted by our company. "O-157" has recently become known as one of the bacteria responsible for foodborne outbreaks, and elucidating the mechanism of its verotoxin production is an urgent task. Bacteria have a cell wall surrounding them, making it difficult for chemicals such as fixatives to penetrate. The sample preparation method is fundamentally similar to that of animal tissues, but the image quality can be improved by extending the processing time and performing fixation at room temperature. 【Analysis Overview】 ■ Target of Analysis: Pathogenic Escherichia coli O-157 ■ Sample Preparation Method: Essentially similar to animal tissues - Image quality is improved by extending processing time and performing fixation at room temperature. *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis

We would like to introduce a case study of the analysis of the "renal glomerulus" conducted by our company. The kidneys are organs that produce urine from blood. In the center of this diagram is a spherical glomerulus that filters urine from blood, and surrounding it are the renal tubules that reabsorb necessary components from the filtrate. [Analysis Overview] ■ Analysis Subject: Renal Glomerulus ■ The kidneys are organs that produce urine from blood ■ There is a spherical glomerulus in the center that filters urine from blood ■ There are renal tubules that reabsorb necessary components from the filtrate *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis

We would like to introduce a case study of the analysis of 'hepatocytes (pig liver)' conducted by our company. The liver has many functions, including lipid and protein synthesis, metabolism and removal of drugs, and energy storage, most of which are composed of hepatocytes. There are also other components such as bile canaliculi (BC) and blood vessels (HS), within which blood cells (EC, Kf) can be observed. The inserted photo shows an enlargement of the cell membranes of adjacent cells, revealing that each cell membrane has a double structure approximately 7nm wide. Additionally, hepatocytes possess typical organelles that are often referenced when explaining the structure and function of general cells. [Analysis Overview] ■ Analysis Subject: Hepatocytes (pig liver) ■ It is evident that each cell membrane has a double structure approximately 7nm wide. ■ Hepatocytes have typical organelles that are frequently cited when explaining the structure and function of general cells. *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis

This document is a collection of case studies summarizing material analysis conducted by Hanai Corporation's Electron Microscope Technology Research Institute. The "Ultra Microtome" is suitable for producing high-quality ultra-thin sections and cross-sections across a wide range of fields, including biological tissues, metal plating layers, inorganic deposition layers, and nanoparticles. Our company has started offering contract analysis services. We will conduct material analysis in collaboration with partner organizations. [Published Cases] - Structural analysis of liposomes - Structural analysis of HIPS - Structural analysis of lens cross-sections - Cross-sectional observation of candy bags - Morphological observation of metal particles using the dispersion method (metal microparticles/metal-supported carbon) *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis
• Electron microscope
• Other microscopes

This document presents analysis cases conducted by Hanai Corporation's Electron Microscope Technology Research Institute. With the "ultramicrotome," it is possible to produce sections ranging from 30nm to 5μm. It is suitable for high-quality ultra-thin sections and cross-sections in a wide range of fields, including biological tissues, metal plating layers, inorganic deposition layers, and nanoparticles. We handle numerous analyses. For more details, please contact us. [Published Cases] ■ Structural analysis of liposomes ■ SEM observation of lactic acid bacteria ■ Analysis of skin lamellar structures *For more information, please refer to the PDF document or feel free to contact us.

• Contract Analysis
• Electron microscope
• Other microscopes

At the Flower Market Electron Microscopy Technology Research Institute, we offer contract services for "Virus Safety Testing" using electron microscopy. Under strict management in compliance with GLP/GMP, we conduct virus safety tests. For example, when producing biopharmaceuticals using CHO cells, it is necessary to perform electron microscopy observations in the retrovirus negative test based on the test items outlined in ICH Q5A and Q5D. Based on our years of accumulated experience and achievements, we provide our customers with highly reliable data. 【Features】 ■ Safety testing using transmission electron microscopy ■ Years of accumulated experience and achievements ■ Providing customers with highly reliable data *For more details, please refer to the PDF materials or feel free to contact us.

• Contract Analysis

We conduct "electron microscope analysis of exosomes." By visualizing with an electron microscope, it is possible to confirm the morphology and size. What we require from you is approximately 50μL of sample. (Sample volume can be discussed.) In the case of culture supernatant, the exosome concentration may be low, so please provide a sample that is as concentrated as possible. For serum, we have a track record of being able to analyze with 50μL, but depending on the sample, there may be a low number of particles. In such cases, we may request additional samples. Please consult us for details. We will provide a preliminary report via email approximately two weeks after receiving the sample. Additional imaging will be conducted as needed. 【Deliverables】 ■ Report and imaging data (imaging data will be delivered on CD-R or DVD-R) *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis
• Electron microscope
• Other microscopes

We offer contract services for structural analysis and morphological observation of nanomaterials such as nanotubes using TEM, SEM, and EDS in the nanoscale region.

• Contract measurement

Observation of multilayer membranes such as liposomes can be performed using negative staining method for TEM observation. Additionally, it can also be done using resin embedding method.

• Contract measurement

We offer fast and high-quality analysis using an electron microscope, from sample preparation to observation and imaging.

• Contract measurement

We analyze the localization of proteins within cells, which cannot be tracked by fluorescence or laser microscopy, using immunoelectron microscopy.

• Contract measurement

【Technical Capability】Conducted by a certified electron microscope technician from the Japan Society of Microscopy. 【Responsiveness】We provide detailed support through a one-stop service. 【Speed】We respond quickly even to advanced analyses. 【Cost Reduction】By outsourcing to us, we contribute to your cost savings. 【Confidentiality】As a completely independent capital entity, there is no leakage to external parties. 【Examples of Contracted Work】 ■ Confirmation of regenerated tissue through observation of cultured tissue ■ Particle measurement of nano-order powders ■ Proof of functional foods through comparative observation of starch granule states ■ Observation of emulsion shapes through negative staining ■ Crystal analysis through ultra-thin slicing of teeth, etc. *For more details, please download the catalog or contact us.

• Contract measurement
• Electron microscope