Notice of the Co-hosted Academic Luncheon Seminar Web Event at the 17th Annual Meeting of the Japanese Society for Regenerative Medicine.

Study on Quality Control through Parameter Measurement in iPS Cell Culture Supernatant

Focusing on "cell proliferation," one of the indicators of cell quality, we compared the results of cell proliferation and culture medium component analysis when changing the culture conditions. This is the result data from an experiment where the frequency of medium exchange was set to three conditions during the maintenance culture of iPS cells in a feeder-free environment, followed by three passages. In this verification, we used the cell culture environment analysis device "BioProfile FLEX2." The "BioProfile FLEX2" is a non-invasive, low-sample-volume device that can measure up to 16 items, including metabolites and cell viability, in just 4 minutes and 30 seconds, or 2 minutes with individual modules. 【Product Specifications】 Analysis Items: Glucose/Lactate/Glutamine/Glutamate/NH4+/Na+/K+ Ca++/pH/pO2/pCO2/Total Cell Density/Viable Cell Density/Viability/Cell Diameter/Osmolality Automatic Calibration: 2 hours (fixed)/Time required 2 minutes Operating Environment: Temperature 10–30°C, Humidity 20–85% 【Features】 ■ Simultaneous analysis of up to 16 items ■ Measurement time: as short as 2 minutes, as long as 4 minutes and 30 seconds (depending on measurement items) ■ Measurement of consumption and production rates of major metabolites ■ Compatible with multiple shapes of sample containers ■ Equipped with an auto-sampling function for continuous measurement

• Other Culture
• Other bio-related products
• Other analytical and testing equipment

Presentation of verification materials exploring the application to the evaluation of cell quality and experimental systems!

This document is a verification report that measures parameters in the culture supernatant during the maintenance culture of iPS cells and the differentiation induction process from iPS cells to dopamine-producing neural progenitor cells, exploring applications for evaluating cell quality and experimental systems. In addition to the experimental content and the composition of reagents and media, it discusses differentiation induction and maintenance culture. 【Contents】 ■ Purpose ■ Experimental Content ■ Reagents and Media Composition - Differentiation induction into neural progenitor cells - iPS cell culture ■ Discussion *For more details, please refer to the PDF document or feel free to contact us.

• others

An automatic analysis device for cell culture environments that achieves "shortened measurement time" and "significant reduction in sample volume"! All 16 items can be measured with just a small amount of sample.

The "BioProfile FLEX2" is an analytical device that utilizes micro-sensor technology. This technology allows the "BioProfile FLEX2" to achieve "reduced maintenance related to electrodes," "shortened measurement time," and "significantly reduced sample volume," making it an automatic analysis device for cell culture environments. It can measure all 16 parameters with a minimal sample volume, completing all 16 measurements in just 4 minutes and 30 seconds, or 2 minutes for individual modules. 【Features】 ■ Monitoring the progress of cell culture ■ Quantification of key components in the culture medium ■ Early detection of abnormalities through regular monitoring ■ Capability to measure multiple parameters with one device, consolidating the functions of multiple devices into one ■ Numerical backing for the timing of medium exchange *For more details, please request documentation or view the PDF data available for download.

• Other physicochemical equipment

We would like to extend our heartfelt congratulations on the continued prosperity of your esteemed company. Our company will be exhibiting at the following trade show, where we will introduce a number of products, including our new product, the Pillow Packaging Full Air Tightness Inspection Machine MSQ-2003. We sincerely apologize for the inconvenience during your busy schedule, but we would like to invite you to visit us at this opportunity. 【 Exhibition Name 】 JAPAN PACK 2025 Japan Packaging Industry Exhibition https://www.japanpack.jp/ 【 Dates 】 October 7 (Tuesday) to October 10 (Friday), 2025 *4 days 10:00 AM to 5:00 PM 【 Venue / Booth Number 】 Tokyo Big Sight / East Hall 8, Booth 505

[Speaker] Yuichi Murakami Director, Automotive Sector, Deloitte Tohmatsu Consulting LLC [Key Lecture Content] In the automotive industry, which is undergoing a once-in-a-century transformation, "SDV: Software Defined Vehicle" will play a central role in this change. However, the term "SDV" encompasses diverse meanings, leading to varying interpretations and resembling a buzzword. In this lecture, we will explore the true digital transformation (DX) in the automotive industry encapsulated by the term "SDV," starting from the changes in the value provided by automobile manufacturers, namely "the car (what is produced)," and including both "changes in how it is made" and "changes in how it is used." We will provide a comprehensive examination of the impact of digital technology on the automotive industry and the points of transformation. [Lecture Topics] 1. Changes in usage - Shifts in user values 2. Changes in what is produced (the car) - Software and digitalization of vehicles 3. Changes in how it is made - Software-based vehicle development 4. Changes in the supply chain 5. Changes in business viability 6. Q&A / Business card exchange

[Lecturers] Masaki Mochimaru, Fellow, National Institute of Advanced Industrial Science and Technology Masato Komiya, CEO, d-strategy, inc. / CEO, Third Ecosystem, inc. Masato Komiya, Specially Appointed Associate Professor, Data Science Research Institute, Tokyo International University [Key Lecture Content] The decrease in the labor population due to declining birth rates and aging society, along with the increasing burden of nursing care and rehabilitation, presents business opportunities. There is growing attention on the approach of "human augmentation," which not only involves complete automation through AI and robots but also enhances human capabilities. In particular, the "interverse" business, where people connect with virtual spaces to transcend time and space constraints and extend value into the real world, is expected to thrive. The lecture will introduce the latest trends through examples of collaboration with companies and national projects. [Lecture Items] 1. Human Augmentation 2. XR 3. Service Transformation in Manufacturing 4. Metaverse 5. Virtual Economy 6. Q&A / Business Card Exchange

[Speaker] Abeam Consulting Co., Ltd. Supply Chain Sustainability Unit Principal Shiro Komatsu Digital Technology Business Unit Artificial Intelligence Leap Sector Senior Expert Miho Yoshida [Key Lecture Content] In response to the rapid changes and complexities in the current business environment, procurement organizations need to break away from their downstream positioning and become a strategic function that leads company-wide reform and corporate value. This seminar will redefine the ideal state that procurement should aim for and the challenges that need to be overcome, introducing the possibilities of business reform utilizing AI agents/solutions along with specific case studies. We will explain the latest AI capabilities such as automated quotation assessment and automated delivery date adjustments, clarifying what your company should do now to strengthen procurement in light of your current situation. [Lecture Items] 1. The ideal state that procurement should aim for and the current situation - Case studies of procurement reform 2. What AI agents/solutions can achieve - Including case study presentations 3. Towards realizing the strategic function of the procurement department 4. Q&A / Business card exchange

[Speaker] Tadakazu Arakawa, Professor Emeritus, The University of Tokyo / Vice President, World Wind Energy Association [Key Lecture Content] After an overview of wind power generation, the latest information on the business trends and technological developments of offshore wind power generation, which has become one of the main power sources, will be explained. Based on the Renewable Energy Sea Area Utilization Act, operators have been determined for offshore wind power projects up to Round 3, and there has been significant progress in expanding into vast Exclusive Economic Zones (EEZ). On the other hand, due to international material shortages and rising prices stemming from issues like the Ukraine crisis, some operators have expressed the possibility of withdrawing from projects. In Japan, where there are no wind turbine manufacturers, discussions will be held on the healthy development and dissemination of offshore wind power towards the carbon neutrality policy by 2050. [Lecture Items] 1. Introduction to Wind Power Generation 2. Current Status of Offshore Wind Power Generation 3. Offshore Wind Power Projects Based on the Renewable Energy Sea Area Utilization Act 4. Technology and Wind Turbines in Offshore Wind Power Generation 5. Project Formation and Regional Promotion of Offshore Wind Power Generation 6. Progress of Japan's Offshore Wind Power Generation Aiming for Carbon Neutrality 7. Q&A / Business Card Exchange