6th Annual Meeting of the Japanese Society for Genome Editing Poster Presentation

Developed through joint research with the National Institute of Infectious Diseases. Achieves determination of the insertion site of foreign DNA in a short time, with high sensitivity and low cost.

The "RAISING method" is a technique for detecting the insertion sites of foreign DNA in the genome. It allows for rapid and reproducible measurement results with a small amount of DNA. It can identify insertion sites of viral genome insertions, viral vector insertions, searches for fusion genes that could be drug discovery targets, and off-target knock-ins in genome editing, among others. 【Features】 - Analysis can be arranged according to the purpose and target. - Multiple insertion sites can be detected in a single analysis. - High reproducibility of results is achieved through proprietary technology. 【Analysis Examples】 - HTLV-1 (Human T-cell Leukemia Virus) Clonality Analysis Leveraging the characteristics of the RAISING method, which can quickly detect with a small amount of DNA, we achieved high detection sensitivity even with a proviral load of 0.032%. - Off-target Detection of Knock-ins in Genome Editing For knock-in mice created using CRISPR/Cas, we conducted detection of knock-in efficiency and off-target sites by combining with NGS. *For details on examples and services, please refer to the materials available for download.

• Contract Analysis
• Contract Analysis
• Analysis Services

We will introduce examples of off-target detection in knock-in during genome editing!

We would like to introduce a case study of our random integration analysis service using the RAIS method. For a knock-in mouse created using CRISPR/Cas, we attempted to detect the knock-in efficiency and off-target sites by combining the RAIS method with NGS. It was suggested that the donor DNA used for the knock-in was inserted not only into the intended chromosome 9 (chr9) but also into another chromosome. 【Random Integration Analysis Service using the RAIS Method Contents】 ■ Sanger Sequencing Plan ■ Next Generation Sequencing (NGS) Plan *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis
• Contract Analysis
• Analysis Services

Free gene editing is possible! Achieving high-efficiency knock-in/knock-out!

The "Genome Craft Series" is a powerful genome editing tool that enables free genetic modification. "Genome Craft Type CT" can be used similarly to sgRNA by utilizing chemically synthesized crRNA and tracrRNA, allowing for the chemical synthesis of sgRNA by splitting a long sgRNA into two parts. We provide suitable tools for achieving high-efficiency knock-in/knock-out in CRISPR/Cas genome editing technology. 【Product Lineup】 ■ Guide RNA ・Genome Craft Type CT ・Genome Craft Type SG ■ Recombinant Cas9 ■ Donor DNA *For more details, please refer to the PDF document or feel free to contact us.

• Other information systems

Features ● Equipped with Intel Core i9-13900E/i5-13500E *Optional upgrade available ● 64GB memory installed *Optional upgrade available (up to 128GB) ● 2.5-inch 3D TLC SSD 512GB installed *Optional upgrade available ● Windows 11 IoT Enterprise installed *Optional upgrade available ● HDMI x1, DP x1, VGA x1 ports available ● Rear I/O side USB 3.2 Gen 2 x4, USB Type-C x1, GbE x2, 2.5GbE x2, RS-232/422/485 x1

Features ● Equipped with Intel Core i9-13900E / i5-13500E *optional change available ● Equipped with 64GB memory *optional change available ● Equipped with 512GB 3D TLC SATA SSD *optional change available ● Equipped with Windows 11 IoT Enterprise *optional change available ● HDMI x2, DP x2 included ● Rear I/O includes USB 3.2 (Gen2) x3, USB 3.2 (Gen1) x2 ● BTO support available from one unit

Features ● Compatible with 12th/13th/14th generation Intel Core i series ● Equipped with 32GB memory and 512GB 3DTLC SSD *Changeable as an option ● Comes with Windows 11 IoT Enterprise *Changeable as an option ● Equipped with 1 HDMI, 1 DP, and 1 VGA port ● Rear I/O includes 4 USB 3.2, 4 LAN, and 1 COM ● Long-term supply model ● BTO options available from a single unit, allowing changes to CPU, memory, etc.

The high-voltage fuse "EVFC-φ38 850V/1000V" is designed to withstand DC850V/1000V and is utilized as a fuse for the main circuit protection of automotive high-voltage applications, including high-voltage battery packs, drive motors, and charging systems for BEVs and HEVs, featuring excellent heat resistance and durability. It is often adopted as an automotive fuse for high-voltage circuits to meet the needs of hybrid and electric vehicles. The EV fuse developed by PEC has been adopted not only by domestic automakers but also by manufacturers worldwide, and it continues to be improved, becoming the global standard for fuses.

Thank you for your continued support. We sincerely apologize for the inconvenience, but Tentecaster Co., Ltd. will be closed for the year-end and New Year holidays during the following period. Holiday Period: December 27, 2025 (Saturday) to January 4, 2026 (Sunday) * We will resume normal business operations on January 5, 2026 (Monday). Inquiries received during the holiday period will be addressed sequentially after we resume operations. We appreciate your understanding and cooperation in this matter. We look forward to your continued patronage in the coming year. Wishing you all health and happiness from the bottom of our hearts.