[Case Study] Determination of Insertion Sites for Animal Cultured Cells and Plant Cells

We have added a case study of random integration analysis using the RAISING method!

We would like to introduce a case study of our random integration analysis service using the RAIS method. We performed the RAIS method on T-DNA taglines inserted into Arabidopsis thaliana using the Agrobacterium method, as well as on mouse cultured cells with retrovirus vectors inserted. We confirmed the insertion site sequences using Sanger sequencing and mapped the decoded sequences obtained from next-generation sequencing analysis to the genome sequence. As a result, it was confirmed that this method is effective for determining insertion sites in animal cultured cells as well as in plant cells. 【Random Integration Analysis Service using the RAIS Method】 ■ Sanger Sequencing Plan ■ Next-Generation Sequencing (NGS) Plan * For more details, please refer to the PDF document or feel free to contact us. * For case studies, please refer to the URL below.

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Developed through joint research with the National Institute of Infectious Diseases. Achieves determination of the insertion site of foreign DNA in a short time, with high sensitivity and low cost.

The "RAISING method" is a technique for detecting the insertion sites of foreign DNA in the genome. It allows for rapid and reproducible measurement results with a small amount of DNA. It can identify insertion sites of viral genome insertions, viral vector insertions, searches for fusion genes that could be drug discovery targets, and off-target knock-ins in genome editing, among others. 【Features】 - Analysis can be arranged according to the purpose and target. - Multiple insertion sites can be detected in a single analysis. - High reproducibility of results is achieved through proprietary technology. 【Analysis Examples】 - HTLV-1 (Human T-cell Leukemia Virus) Clonality Analysis Leveraging the characteristics of the RAISING method, which can quickly detect with a small amount of DNA, we achieved high detection sensitivity even with a proviral load of 0.032%. - Off-target Detection of Knock-ins in Genome Editing For knock-in mice created using CRISPR/Cas, we conducted detection of knock-in efficiency and off-target sites by combining with NGS. *For details on examples and services, please refer to the materials available for download.

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The glass pot lid has a hydrophilic heat-resistant coating, allowing you to check the cooking state without any fogging.

Based on practical experience in support companies and business companies, SBS Marketing Co., Ltd. provides consulting services related to marketing, sales promotion, and customer attraction mainly in the BtoB (business-to-business) sector. On August 7, 2025 (Thursday), they published a page titled "Can You Leave an 'Impact' in Business Meetings and Presentations!? 'Echoic Memory'". 'Echoic Memory' is the temporary storage of auditory information received through the ears. The page explains the mechanisms and examples of its occurrence, the differences from 'Iconic Memory', points to maximize its effectiveness, and advanced utilization techniques. (Page Overview: Excerpts) ■ What is 'Echoic Memory'? ■ The mechanism of 'Echoic Memory' and familiar examples ■ 'Echoic Memory' and 'Iconic Memory' ■ Examples of utilization in business scenes ■ Four points to maximize the effectiveness of 'Echoic Memory' ■ Advanced utilization techniques for 'Echoic Memory' (DL content only) ▼ For more details, please visit this page. https://sbsmarketing.co.jp/blog/echoic-memory-2025-08/

We would like to inform you that our company has recorded a special loss (executive retirement benefits) for the first half of the fiscal year ending December 2025, as detailed below. Furthermore, the payment of executive retirement benefits was approved at the "51st Annual General Meeting of Shareholders" held on March 28, 2025. 1. Recording of Special Loss In recognition of the long-standing contributions of one retiring director, we have recorded a special loss of 14,045 thousand yen as executive retirement benefits. 2. Impact on Performance The impact of this matter on the performance forecast for the fiscal year ending December 2025 is minimal. End.

We will disclose the interim financial results for the fiscal period ending December 2025.

As part of the "Hometown Ibara Future Creation Human Resource Development Project," we welcomed local elementary and middle school students to the special summer program "Ibara Job Exploration Tour 2025," a collaboration between the Ibara City Chamber of Commerce and the Corporate Branding Committee. We conducted a presentation that clearly and enjoyably introduced how our technology supports society, allowing the children to learn about the appeal of manufacturing and the significance of working while interacting with actual devices and products. Through this initiative, we hope to deepen our connection with the community and nurture the curiosity of the children who will carry the future. We sincerely thank everyone who participated, as well as all those involved in planning and organizing the event.