No need for gel staining or decolorization! A fluorescent labeling kit that allows for immediate detection of proteins after electrophoresis.
The WSE-7010 EzLabel FluoroNeo is a kit that allows for the fluorescent labeling of amino groups simply by mixing it with a protein sample solution and heating it before electrophoresis. There is no need for cumbersome gel staining or decolorization; after electrophoresis, detection and imaging can be done immediately by exciting with a blue LED or UV light, which is effective for improving experimental efficiency and reducing time. The gel can also be analyzed by Western blotting after observation. 【Features】 No staining or decolorization needed; detection can be done immediately after electrophoresis! Easy operation! Western blotting is possible! ★For more details, please download the catalog or contact us.
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basic information
EzLabel FluoroNeo fluorescently labels amino groups simply by mixing it with a protein solution and heating (reacts at 37 to 95°C). It allows for fluorescent labeling just by replacing the electrophoresis preparation solution (sample buffer) with this product, enabling immediate detection and imaging after electrophoresis using a Blue LED or UV light. No staining or decolorization steps are necessary. With excitation from a Blue LED, detection can be performed without removing the gel from the glass plate, allowing for high-sensitivity detection at silver staining levels. The molecular weight remains almost unchanged even after labeling. After detection (observation), it can also be used for Western blot analysis (detection on membranes, reaction with antibodies). The price is offered reasonably, similar to staining reagents.
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Applications/Examples of results
☆ Stain-free protein electrophoresis! Detect immediately after electrophoresis without staining or decolorization ☆ Sample preparation for SDS-PAGE Fluorescent labeling of proteins and polypeptides Protein electrophoresis (SDS-PAGE, two-dimensional electrophoresis) Western blotting Immunoprecipitation, etc.
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Company information
ATTO is a company that develops, manufactures, and sells scientific instruments for research, such as proteins and nucleic acids. Its traditional representative products include "Peristaltic Pump," "Mini-Slab Electrophoresis Device," "Pajel," and "Print Graph," which are used in research laboratories in Japan and abroad. In the current era of advanced protein research, ATTO is developing innovative and powerful products such as the new tools for gene expression analysis: "Cell Graph," "AB-2550 Chronos," "AB-2250 Luminescence Sensor MCA," and "CLuc."