You can freely adjust the oxygen concentration.
No need for CO2 incubators or gas cylinders such as carbon dioxide, nitrogen gas, or mixed gases. The O2 concentration inside the pouch can be adjusted to 0.1-15% for cultivation.
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basic information
To culture cancer cells, a low-oxygen environment is essential, and until now, elaborate equipment has been required, including specialized CO2 incubators and gas cylinders for nitrogen and mixed gases in addition to carbon dioxide. Despite their high initial and running costs, these setups typically only allow for a maximum of 0.5% oxygen, and in many cases, can only reach and maintain around 2%. The low-oxygen culture kit enables easy, convenient, rapid, and low-cost low-oxygen culturing. By using gas barrier pouches and gas concentration adjusting agents, it allows for the adjustment of the desired O2 concentration within a range of 0.1% to 15% during cultivation.
Price information
46,000 yen and up
Price range
P2
Delivery Time
P1
Applications/Examples of results
1. Place the "O2 meter" deep inside the "gas barrier pouch" and position a petri dish filled with culture medium right next to it. 2. Open the aluminum bag of the "gas concentration adjusting agent," take out the inner bag, and place it near the entrance along line A in the diagram below. 3. Seal line B using a "clip," and the O2 inside the pouch will be absorbed in just a few minutes, creating a low-oxygen environment. 4. Monitor the scale of the O2 meter, and when it approaches the desired O2 concentration after several minutes, replace the air inside the petri dish with the air inside the pouch. After that, if the O2 concentration drops slightly below the desired level, seal line A with a clip to stop O2 absorption. 5. If the O2 concentration inside the pouch becomes too high or too low compared to the desired concentration, some additional operations will be necessary. Please consult us. 6. The equipment used for this culture method, except for the "gas concentration adjusting agent," can be reused, keeping both initial and running costs extremely low.
Company information
As we enter the 21st century, the development of science and technology, including information technology (IT) and biotechnology, is accelerating, presenting new possibilities for people's lives. On the other hand, issues such as environmental pollution caused by chemical substances and the emergence and resurgence of infectious diseases due to microorganisms have not been overcome even with the latest scientific technologies, and are instead becoming more serious and complex. In this situation, the importance of the work of researchers, engineers, and healthcare professionals tackling these problems that threaten people's health will continue to grow. We aim to contribute to these individuals through the provision of instruments and consumables in the fields of environmental analysis, measurement, and microbiological research. We will reflect on what we can do and what we can uniquely offer, striving to provide unique value.
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