Achieving high survival rates and high transfection efficiency for primary cells (primary cultured cells) and immune/blood system cells!
The Super Electroporator NEPA21 Type II has added a damping rate setting function to its 4-step multi-pulse method, achieving high survival rates and high transfection efficiency even for primary cells, which are said to be difficult for gene introduction, as well as immune and blood cells. Additionally, it does not require expensive dedicated reagents or buffers, making it very economical with minimal running costs. 【Gene introduction is possible for various targets such as the following】 ■ Cultured cells (suspension cells) ■ Adherent cultured cells ■ ex vivo tissues (brain slice sections, pancreatic islets, etc.) ■ in vivo mice and rats (muscle, liver, skin, testes, ovaries, brain, retina, etc.) *For more details, please request materials or view the PDF data available for download.
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basic information
【Features】 ○ Reduces damage to cells and significantly improves introduction efficiency ○ 4-step multi-pulse method + attenuation rate setting function (0-99%) ○ Displays not only the current values of all output pulses but also the energy (joules) For more details, please contact us or download the catalog.
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Delivery Time
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Applications/Examples of results
**Applications** ○ In Vitro → Cubic electrodes: High transfection efficiency and high survival rate for both primary and established cells. No expensive dedicated reagents or buffers are required. → Electrode with legs for adherent cells: High-efficiency gene introduction to cells in an adherent state (while still attached). ○ In Vivo → Direct gene introduction into living tissues of mice, rats, and other organisms, as well as plant seeds (muscle, liver, skin, testes, ovaries, eyeball, bladder, kidneys, brain, retina, cornea). ○ In Utero (Exo Utero) → Direct gene introduction into the brain ventricles of fetal mice and rats in utero. ○ In Ovo (Ex Ovo) → Direct gene introduction into chick embryos (chicken embryos). ○ Ex Vivo → Direct gene introduction into tissue slices (such as brain), excised organs, and whole embryo cultures (mouse and rat fetuses). ● For more details, please contact us or download the catalog.
Detailed information
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Primary BMMC Survival rate 80%
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Primary BMMC Implementation Efficiency 83%
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Company information
Neppajine takes pride in contributing to the peace and progress of humanity through biotechnology as a specialized company in life science research equipment, including electroporation (gene introduction using electric pulses) and sonoporation (gene introduction using ultrasound). We believe it is our mission to provide excellent products and information not only within Japan but also to universities, research institutes, hospitals, and private companies around the world. In practice, we have numerous products in use, including gene introduction devices, cell fusion devices, and ultrasound gene introduction devices. We continuously absorb cutting-edge knowledge and technology in biotechnology, engage in close sales activities with our users, provide the latest products, technologies, and information, and respond with a rapid maintenance system. Towards the development of humanity and a bright, happy future, Neppajine will continue to move forward strongly alongside biotechnology.