Correlation comparison using the overlay of refractive index images and fluorescence images, rapid measurement in just a few seconds.
The 3D Cell Explorer-fluo, a holographic and fluorescence microscope for cell observation, is an innovative device that combines 3D refractive index imaging and 2D fluorescence imaging using holography and tomography technologies. It allows for rapid and easy observation of the internal structures and organelle distributions of cells and tissues. By overlaying fluorescence images of various cellular organelles and cell membranes that have been fluorescently labeled with refractive index images, it is possible to calibrate the digital staining to match the staining images of the fluorescent labels. By applying this calibrated digital staining to the 3D refractive index image, the 2D fluorescence images can be extended into 3D images, visualizing the three-dimensional distribution. This calibration process allows for a correlation between fluorescence staining and digital staining, enabling compatibility with unstained samples. The constructed 3D images can be enlarged, reduced, rotated, and displayed in cross-sections, allowing for comprehensive observation of the interior of cells from various perspectives.
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Features ■ Correlation comparison through overlay of 3D images and fluorescence images is possible ■ Calibration of digital staining for various organelles using fluorescence images ■ Rapid measurement within 3 seconds (during refractive index imaging) ■ In addition to 3D imaging of cells, 4D imaging (over time) is also possible ■ Simultaneous staining with up to 10 types: digital staining (7 types), fluorescence staining (3 types) ■ High-resolution imaging at the nanometer scale, exceeding the diffraction limit of light △x,y 200nm, △z 500nm
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Uses ● Cell division ● Monitoring of cell morphology ● Cell differentiation ● Cell-to-cell interaction ● Intracellular movement ● Cell remodeling processes ● Cell death (apoptosis, necrosis) ● Drug monitoring ● In vitro fertilization and so on
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| Model number | overview |
|---|---|
| 3D Cell Explorer-fluo | Supports measurement of refractive index images (3D) and fluorescence images (2D) |
| 3D Cell Explorer | Supports measurement of refractive index images (3D) |
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We are a group of experts specializing in spectroscopy, focusing on cutting-edge equipment such as light sources, spectrometers, detectors, our unique laser measurement systems, analytical devices, and femtosecond laser processing.
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