Acquisition of the Certificate of Material Suitability for Regenerative Medicine Products! Serum-free culture of T lymphocytes is possible.
"iMediam for T" is a culture medium for human T lymphocytes. With our special blend based on our expertise, it effectively promotes the proliferation of T lymphocytes. 【Features】 ■ Obtained a certificate of suitability for materials used in regenerative medicine products ■ Serum-free culture of T lymphocytes is possible ■ Free from xenogeneic components ■ Manufactured in a GMP-compliant facility *For more details, please refer to the PDF document or feel free to contact us.
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basic information
【Specifications】 ■Applicable: Human T lymphocytes ■Storage conditions: Cool, dark place (2-10°C) ■Sterility testing - Endotoxin: Colorimetric method - Mycoplasma: Culture method - Fungi, bacteria: Surface spread method on Canten plates *For more details, please refer to the PDF document or feel free to contact us.
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【Purpose】 ■ Human T lymphocyte culture *For more details, please refer to the PDF document or feel free to contact us.
Detailed information
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Comparison of the performance of our currently used serum-containing medium and iMediam for T (Day 14 of culture) Peripheral blood mononuclear cells (PBMCs) were isolated from the blood of 9 healthy individuals who provided informed consent, and a comparative culture was conducted using our serum-containing medium and iMediam for T (serum-free). Cultures were maintained in OKT3 antibody-coated flasks for the first 5 days, after which they were transferred to gas-permeable culture bags, resulting in a total culture period of 14 days. The final liquid volume was set at 2,000 mL.
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Surface antigen analysis After culturing PBMCs from three healthy individuals who provided informed consent in iMediam for T (serum-free) for 14 days, the proportion of CD3-positive cells, which are indicative of T lymphocytes, was measured using a flow cytometer. In all three samples, the proportion of CD3 was over 98%, indicating that T lymphocytes are selectively proliferating.
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Our company, taking advantage of the enforcement of the regenerative medicine-related law, is starting a "contract processing business" for tissues and cells as a new partner for medical institutions engaged in regenerative medicine and companies developing regenerative medicine-related products, based on the technologies and achievements we have cultivated so far. We are also expanding our business centered on cell processing technology. If you are having trouble with the processing and storage of activated lymphocytes, please feel free to contact us.