Tissue samples are taken from the obtained offspring! They are produced by microinjection into fertilized eggs.
Our company is engaged in the production of "transgenic mice." Transgenic mice are created by purifying DNA fragments of the expression unit that includes a promoter, the gene to be expressed, and a polyA sequence from plasmid DNA, and then microinjecting them into fertilized eggs. From the resulting offspring, tissue samples are collected, and genotyping PCR is performed to investigate the insertion of the injected gene into the genome, thereby identifying the founder mouse (FO). [Features] - Production by injecting the gene expression unit into fertilized eggs of the C57BL/6 strain - Forced expression of the gene in mouse individuals - Specific expression can be achieved by selecting the promoter - Strong expression throughout the body is also possible by using the CAG promoter *For more details, please refer to the PDF materials or feel free to contact us.
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[Work Process] ■ Extraction and purification of expression units from plasmid DNA ■ Injection into 200 C57BL/6 strain fertilized eggs ■ Rearing of offspring until 8 weeks of age ■ Collection of tissue samples from offspring ■ Setting PCR conditions for genotyping ■ Genotyping PCR of offspring *For more details, please refer to the PDF document or feel free to contact us.
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For more details, please refer to the PDF document or feel free to contact us.
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Our company is engaged in the production of gene knockout mice, providing genetic information to research institutions such as universities and pharmaceutical companies, selling reagents, and conducting non-clinical and clinical trial contracts, as well as pathological diagnosis. Through the development of biological resources from individual organisms to genomes, we provide genetic information for foundational research and in the fields of medicine and healthcare. Please leave the production and sale of knockout mice, gene knockout mice, and antibodies to us.