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  6. Genome Editing Tool 'GenomeCraft - Donor DNA'

Genome Editing Tool 'GenomeCraft - Donor DNA'

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last updated:Apr 06, 2021

ファスマック
ファスマック
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We will introduce ssODN synthesis, ssDNA production, and targeting vector creation!

We would like to introduce our genome editing tool, 'GenomeCraft - Donor DNA.' In "ssODN (single-stranded oligodeoxynucleotide donor DNA) synthesis," it can be used as donor DNA to knock in sequences of up to several dozen bases, including base substitutions and loxP sites. In "ssDNA (single-stranded DNA) production," we create long single-stranded DNA that cannot be synthesized using oligodeoxynucleotide synthesis, and in "targeting vector production," we produce donor DNA for gene targeting, such as gene knockout and knock-in. Please feel free to contact us when you need our services. 【Service Details】 ■ ssODN (single-stranded oligodeoxynucleotide donor DNA) synthesis ■ ssDNA (single-stranded DNA) production ■ Targeting vector production *For more details, please refer to the PDF document or feel free to contact us.

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Genome Editing Tool 'GenomeCraft - Donor DNA'

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  • Related Link - http://fasmac.co.jp/genome_editing_donordna

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【Service Details】 ■ ssODN (single-stranded oligodeoxyribonucleic acid) synthesis - By adding approximately 40 to 80 nucleotides homologous to the CRISPR/Cas target region at both ends of the knock-in sequence to be introduced, it functions as donor DNA for homologous recombination. ■ ssDNA (single-stranded DNA) production - Using high-fidelity amplification products from cloned DNA confirmed to have no mutations, we deliver high-precision single-stranded DNA. ■ Targeting vector production - Enables visualization through the introduction of reporter genes and conditional expression control through the introduction of gene cassettes, greatly expanding the scope of experiments. *For more details, please refer to the PDF document or feel free to contact us.

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Genome editing tool 'GENOME CRAFT RNA'

Genome editing tool 'GENOME CRAFT RNA'

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Fasmac Inc. "Technical Introduction Materials - New Added Value to Existing Platforms -"

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Long-chain RNA synthesis (mRNA contract synthesis service)

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We will synthesize your desired long-chain RNA using in vitro transcription methods. This can be used for various applications such as RT-PCR amplification control RNA, gRNA for genome editing, small RNA, mRNA preparation, RNA aptamers, etc. The synthesis will be performed via in vitro transcription using T7 polymerase from template DNA (prepared by PCR from plasmid clones or oligo DNA). After silica column purification, we will confirm purity and yield through absorbance measurement and electrophoresis. 【Details】 ■ Length: 50–120 nt ■ Guaranteed delivery amount: 20 μg ■ Template DNA: Synthetic oligo DNA (no additional cost) ■ Delivery time: 10 business days ■ Length: 121–1500 nt ■ Guaranteed delivery amount: 40 μg ■ Template DNA: Artificial gene synthesis (additional cost) ■ Delivery time: 10 business days (after receipt of template DNA) If you have a plasmid clone to use as a template, that can also be utilized. If you have requests for longer RNA, increased delivery amounts, 5' cap, polyA-tail, etc., please feel free to consult with us.

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ファスマック

ファスマック

Pharmaceuticals and Biotechnology

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In recent years, advancements in molecular biology have led to remarkable progress in research such as the determination of the complete genome sequences of organisms and subsequent gene function analysis. Along with these technological advancements, molecular biological techniques such as nucleotide sequence analysis, real-time PCR, and DNA chips have become widely utilized in the field of food analysis. Since its establishment in 2001, Fasmak Co., Ltd. has been developing technologies for the "Japanese Standard Analysis Method" for genetically modified foods and food allergens in collaboration with related agencies of the Ministry of Agriculture, Forestry and Fisheries and the Ministry of Health, Labour and Welfare. The developed testing technologies are provided not only in Japan but also in the United States, China, and other countries. Additionally, Fasmak has been actively engaged in international standardization activities for "food inspection methods using molecular biological techniques" since its establishment, and its technical capabilities are internationally recognized. Furthermore, in partnership with Eurofins Scientific, one of the world's largest testing companies, Fasmak is advancing the introduction of the high technical capabilities possessed by the Eurofins Group. Moving forward, Fasmak will continue to provide world-class new testing technologies to everyone.

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