Long-chain RNA synthesis (mRNA contract synthesis service)

Synthesis of long-chain RNA using in vitro transcription method! Plasmid clones can also be used as templates.

At Fasmac, we offer "long-chain RNA synthesis." We synthesize RNA through in vitro transcription using T7 polymerase from template DNA (prepared from plasmid clones via PCR or oligo DNA), followed by purification with silica columns. The purity and yield are confirmed through absorbance measurement and electrophoresis. Please contact us with your desired sequences for various applications such as RT-PCR amplification control RNA, gRNA for genome editing, small RNA, mRNA preparation, RNA aptamers, etc. 【RNA Usage Examples】 ■ RNA of the GFP gene sequence was synthesized, and after adding a 5' cap and polyA-tail, it was introduced into cultured cells using the lipofection method. ■ Green fluorescence was observed approximately 5 hours after introduction. *For more details, please refer to the PDF materials or feel free to contact us.

• Contract Analysis
• Contract Analysis
• Analysis Services

We offer a variety of research support applications that allow for approaches tailored to different research stages. Please feel free to contact us for more information.

<Strengths and Features of Fasmac Co., Ltd.> ■ World-class inspection and manufacturing technology ISO17025 certification, ISTA certification, ISO9001 certification ■ Commitment to research and development Standard analysis methods for GM foods, GM seed screening technology, and many others ■ Robust infrastructure and sales channels Various DNA analysis devices, numerous skilled technicians Established sales network to various research institutions <Research and Development Achievements (Partial)> ■ Improvement of genome editing technology CRISPR/Cas method Joint research with Tokyo Medical and Dental University, Hiroshima University, and Keio University ■ Random integration analysis Joint research with the National Institute of Infectious Diseases ■ Molecular quantity guaranteed DNA standard materials Joint research with Ricoh Company, Ltd. ■ Development of a skin resident bacteria detection kit using DNA chromatography methods Joint research with Menard Co., Ltd. *If you have any concerns, please feel free to contact us. We aim to add new value to existing platforms and hope to collaborate with all of you.

• Contract Analysis
• Contract Inspection
• others

We will introduce ssODN synthesis, ssDNA production, and targeting vector creation!

We would like to introduce our genome editing tool, 'GenomeCraft - Donor DNA.' In "ssODN (single-stranded oligodeoxynucleotide donor DNA) synthesis," it can be used as donor DNA to knock in sequences of up to several dozen bases, including base substitutions and loxP sites. In "ssDNA (single-stranded DNA) production," we create long single-stranded DNA that cannot be synthesized using oligodeoxynucleotide synthesis, and in "targeting vector production," we produce donor DNA for gene targeting, such as gene knockout and knock-in. Please feel free to contact us when you need our services. 【Service Details】 ■ ssODN (single-stranded oligodeoxynucleotide donor DNA) synthesis ■ ssDNA (single-stranded DNA) production ■ Targeting vector production *For more details, please refer to the PDF document or feel free to contact us.

• Contract Analysis
• Contract Analysis
• Analysis Services

The inspection technology is available for everyone not only in Japan but also in the United States, China, and other countries!

At Fasmac, since our establishment in 2001, we have been advancing the development of "Japanese Standard Analytical Methods" for genetically modified foods and food allergens in collaboration with related agencies of the Ministry of Agriculture, Forestry and Fisheries and the Ministry of Health, Labour and Welfare, utilizing molecular biological techniques. The developed testing technologies are available not only in Japan but also in the United States, China, and other countries. Additionally, since our establishment, we have been actively engaged in activities such as the international standardization of "food inspection methods using molecular biological techniques," and our technical capabilities are internationally recognized. 【Business Activities】 ■ Food inspection and various contract services ■ Manufacturing and sales of food inspection reagents, manufacturing and sales of reagents for genetic engineering *For more details, please refer to the PDF materials or feel free to contact us.

• Contract Inspection
• Other contract services

● This is a leak testing device for small electronic components that does not use fluorinated inert liquids (PFAS-free). ● There is no longer a need for unstable visual confirmation through liquid immersion testing. It adopts a quantitative testing method that allows for stable pressure leak testing. ● It is a tabletop leak testing device specifically for gross leaks, with accuracy equal to or greater than that of liquid immersion testing. ● The detectable range is approximately 1×10⁻² Pa·m³/s to 1×10⁻⁶ Pa·m³/s (varies depending on the target product). ● It is suitable for sealed structures ranging from a few millimeters to a maximum of 80 × 70 mm. Please feel free to contact us for more information!

● The production time for casting prototypes can be shortened! Since model production is not required, the time needed to create prototypes, which previously took about a month, can be significantly reduced. ● Model costs can be reduced! By directly producing sand molds from 3D data, there is no need for model production costs. If changes to the model are necessary, they can be addressed by modifying the 3D data. ● Similar products with different shapes and dimensions can be prototyped simultaneously! With one molding process, different products with similar shapes or varying dimensions can be molded at the same time. This allows for the verification of multiple types in a short period.

The "Mechanical Stitching Method" by Nichinichisui Road Machinery Co., Ltd. is a new technique that allows for the repair of metal cracks without the application of heat. By using special bolts and reinforcement plates, cracks are physically removed, and repairs are made without causing any thermal impact to the base material. Repairs can be carried out in environments where open flames cannot be used. Since no heat is applied, the disassembly of equipment is minimized. ◎ For more details, please contact us. ◎ If you require a detailed technical explanation, please contact us.

The "Mechanical Stitch Method" by Nichinichisui Road Machinery Co., Ltd. is a new technique that allows for the repair of metal cracks without the application of heat. By using special bolts and reinforcement plates, cracks are physically removed, and repairs are made without causing any thermal impact to the base material. Repairs can be carried out in environments where open flames cannot be used. Since no heat is applied, the disassembly of equipment is minimized. ◎ For more details, please contact us. ◎ If a detailed technical explanation is needed, please contact us.

The "Mechanical Stitching Method" by Nichinichisui Road Machinery Co., Ltd. is a new technique that allows for the repair of metal cracks without the application of heat. By using special bolts and reinforcement plates, cracks are physically removed, and repairs are made without causing any thermal impact on the base material. Repairs can be carried out even in environments where open flames cannot be used. Since no heat is applied, the disassembly of equipment is kept to a minimum. ◎ For more details, please contact us. ◎ If a detailed technical explanation is needed, please contact us.