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  3. ファスマック
  4. Long-chain RNA synthesis (mRNA contract synthesis service)
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  • Jan 18, 2022
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Jan 18, 2022

Long-chain RNA synthesis (mRNA contract synthesis service)

ファスマック ファスマック
We will synthesize your desired long-chain RNA using in vitro transcription methods. This can be used for various applications such as RT-PCR amplification control RNA, gRNA for genome editing, small RNA, mRNA preparation, RNA aptamers, etc. The synthesis will be performed via in vitro transcription using T7 polymerase from template DNA (prepared by PCR from plasmid clones or oligo DNA). After silica column purification, we will confirm purity and yield through absorbance measurement and electrophoresis. 【Details】 ■ Length: 50–120 nt ■ Guaranteed delivery amount: 20 μg ■ Template DNA: Synthetic oligo DNA (no additional cost) ■ Delivery time: 10 business days ■ Length: 121–1500 nt ■ Guaranteed delivery amount: 40 μg ■ Template DNA: Artificial gene synthesis (additional cost) ■ Delivery time: 10 business days (after receipt of template DNA) If you have a plasmid clone to use as a template, that can also be utilized. If you have requests for longer RNA, increased delivery amounts, 5' cap, polyA-tail, etc., please feel free to consult with us.
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Long Chain RNA Contract Synthesis
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Long-chain RNA synthesis (mRNA contract synthesis service)

Synthesis of long-chain RNA using in vitro transcription method! Plasmid clones can also be used as templates.

At Fasmac, we offer "long-chain RNA synthesis." We synthesize RNA through in vitro transcription using T7 polymerase from template DNA (prepared from plasmid clones via PCR or oligo DNA), followed by purification with silica columns. The purity and yield are confirmed through absorbance measurement and electrophoresis. Please contact us with your desired sequences for various applications such as RT-PCR amplification control RNA, gRNA for genome editing, small RNA, mRNA preparation, RNA aptamers, etc. 【RNA Usage Examples】 ■ RNA of the GFP gene sequence was synthesized, and after adding a 5' cap and polyA-tail, it was introduced into cultured cells using the lipofection method. ■ Green fluorescence was observed approximately 5 hours after introduction. *For more details, please refer to the PDF materials or feel free to contact us.

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<Research Support> Would you like to solve the challenges you are currently facing together?

We offer a variety of research support applications that allow for approaches tailored to different research stages. Please feel free to contact us for more information.

<Strengths and Features of Fasmac Co., Ltd.> ■ World-class inspection and manufacturing technology ISO17025 certification, ISTA certification, ISO9001 certification ■ Commitment to research and development Standard analysis methods for GM foods, GM seed screening technology, and many others ■ Robust infrastructure and sales channels Various DNA analysis devices, numerous skilled technicians Established sales network to various research institutions <Research and Development Achievements (Partial)> ■ Improvement of genome editing technology CRISPR/Cas method Joint research with Tokyo Medical and Dental University, Hiroshima University, and Keio University ■ Random integration analysis Joint research with the National Institute of Infectious Diseases ■ Molecular quantity guaranteed DNA standard materials Joint research with Ricoh Company, Ltd. ■ Development of a skin resident bacteria detection kit using DNA chromatography methods Joint research with Menard Co., Ltd. *If you have any concerns, please feel free to contact us. We aim to add new value to existing platforms and hope to collaborate with all of you.

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Genome Editing Tool 'GenomeCraft - Donor DNA'

We will introduce ssODN synthesis, ssDNA production, and targeting vector creation!

We would like to introduce our genome editing tool, 'GenomeCraft - Donor DNA.' In "ssODN (single-stranded oligodeoxynucleotide donor DNA) synthesis," it can be used as donor DNA to knock in sequences of up to several dozen bases, including base substitutions and loxP sites. In "ssDNA (single-stranded DNA) production," we create long single-stranded DNA that cannot be synthesized using oligodeoxynucleotide synthesis, and in "targeting vector production," we produce donor DNA for gene targeting, such as gene knockout and knock-in. Please feel free to contact us when you need our services. 【Service Details】 ■ ssODN (single-stranded oligodeoxynucleotide donor DNA) synthesis ■ ssDNA (single-stranded DNA) production ■ Targeting vector production *For more details, please refer to the PDF document or feel free to contact us.

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Fasmac Inc. Company Profile

The inspection technology is available for everyone not only in Japan but also in the United States, China, and other countries!

At Fasmac, since our establishment in 2001, we have been advancing the development of "Japanese Standard Analytical Methods" for genetically modified foods and food allergens in collaboration with related agencies of the Ministry of Agriculture, Forestry and Fisheries and the Ministry of Health, Labour and Welfare, utilizing molecular biological techniques. The developed testing technologies are available not only in Japan but also in the United States, China, and other countries. Additionally, since our establishment, we have been actively engaged in activities such as the international standardization of "food inspection methods using molecular biological techniques," and our technical capabilities are internationally recognized. 【Business Activities】 ■ Food inspection and various contract services ■ Manufacturing and sales of food inspection reagents, manufacturing and sales of reagents for genetic engineering *For more details, please refer to the PDF materials or feel free to contact us.

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ファスマック
Pharmaceuticals and Biotechnology
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Truck berth vehicle detection

It's not too late to address the "Logistics 2024 Problem." Why not immediately reduce drivers' waiting times?

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Are you making progress on measures for the "2024 Problem"? With stricter management of drivers' working hours, reducing "waiting time" has become an urgent issue in the logistics field. To address this challenge, our company, Hotron, proposes the "Vehicle Detection Ultrasonic Sensor 'HM-UX2'," which instantly grasps the availability of loading bays. The 'HM-UX2' contributes to the resolution of waiting times by accurately understanding the availability situation, reducing unnecessary waiting and queueing, and shortening waiting times to alleviate the burden on drivers! By replacing the "eyes" of your loading bay management with this high-precision sensor, why not start addressing the "2024 Problem"? Take a look at the product features: "Can it accurately detect availability?" "Can we really use it in our company?" "I just want to know the cost, even if it's an estimate." If you have any questions or requests, please feel free to consult with us.

Aug 08, 2025

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Hidetoshi Tojo appointed as the representative executive president of the U.S. Anthropic Japan Corporation.

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Tokyo, August 7, 2025 - (JCN Newswire) - Anthropic, an AI research and development company that develops the cutting-edge AI model Claude, announced today the appointment of Hidetoshi Tojo as the Representative Executive Officer and President of its Japanese subsidiary. Tojo has extensive experience driving growth for technology companies in Japan, most recently serving as the President and Executive Officer of Snowflake's Japanese subsidiary, where he was involved from the launch of the business and achieved significant growth. He has also contributed to the advancement of data utilization in major Japanese companies by closely collaborating with various system integrators. Additionally, he has experience leading teams at Google Cloud Japan and Microsoft, both at their U.S. headquarters and Japanese subsidiaries. For more details, please refer to: https://acnnewswire.com/press-release/japanese/101731/

Aug 07, 2025

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◆Asano Corporation◆ Notice of Summer Vacation ★ Asano is a development support manufacturer for automobiles, motorcycles, bicycles, aircraft, space rockets, and more.

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We sincerely apologize for the inconvenience, but Asano Co., Ltd. will be closed for summer vacation during the following period. We appreciate your understanding. Closure Period: August 13, 2025 (Wednesday) to August 17, 2025 (Sunday) Reopening: August 18, 2025 (Monday) <Inquiries> We will respond sequentially starting from August 18, 2025 (Monday). Asano Co., Ltd. is a manufacturer that supports the development of mobility parts, including automobiles, motorcycles, bicycles, aircraft, and space rockets. We conduct in-house integrated production of sheet metal, plastic, and composite products. We offer proposals for hybrid molding and multi-material bonding, among others. ■ Inquiries ■ Asano Co., Ltd. Headquarters and Gunma Factory 2718-1 Sanwa-cho, Isesaki City, Gunma Prefecture, 372-0011 TEL: 0270-75-1700 FAX: 0270-75-1600 MAIL: salesg@asano-japan.com Official YouTube Channel: https://www.youtube.com/@asano-japan

Aug 07, 2025

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Kit 1 θ/2 Method Contact Angle Analysis Software

New Product: Simple Contact Angle Meter Kit - Amazon Exclusive Model

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Newly released: Contact Angle Meter Entry Mini Kit Model, Amazon Exclusive Model JISR3257 compliant, includes θ/2 method and contact angle analysis software. Windows PC sold separately. Complete product priced at ¥399,300 (tax included). At the same time, we have started selling individual components for the contact angle meter kit as an Amazon exclusive model: "θ/2 Method Contact Angle Analysis Software" Kit 1, "Stage + Backlight + Housing" Kit 2, and "Dedicated USB Camera" Kit 3. The contact angle meter can be integrated into the device, allowing for the combination of "θ/2 Method Contact Angle Analysis Software" Kit 1 and "Dedicated USB Camera" Kit 3 for applications. Only the necessary elements such as software and camera can be combined. These models are exclusive to Amazon and will be sold and shipped by Amazon.co.jp.

Aug 07, 2025

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Our employee was featured in a special article in the Sanyo Shimbun.

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Our company, NICS Corporation (Headquarters: 1-11-1 Uno, Tamano City, Okayama Prefecture, President: Shinichiro Yamane), had an employee featured in the special article "New Star ★ Running" in the local economy section of the Sanyo Shimbun dated August 7.

Aug 07, 2025

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