No need for DNA fragment preparation! It is possible to connect sequences with promoter activity upstream of the target gene using only PCR.
The forward primer of the present invention does not require complicated operations such as restriction enzyme treatment or ligation, nor the preparation of DNA fragments containing promoter sequences. It is possible to link a sequence with promoter activity upstream of the target gene using only PCR. Even if a promoter sequence is already linked upstream of the target gene, if the expression of the target gene is low, the expression of the target gene can be enhanced by linking a sequence with promoter activity upstream of the target gene sequence or the existing promoter sequence. 【Research Overview】 - No need for complicated operations such as restriction enzyme treatment or ligation, nor the preparation of DNA fragments containing promoter sequences. - It is possible to link a sequence with promoter activity upstream of the target gene using only PCR. - Linear double-stranded DNA with a sequence containing promoter activity can be easily produced upstream of the target gene. - By linking sequences, the expression of the target gene can be enhanced. *For more details, please refer to the PDF document or feel free to contact us.
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For more details, please refer to the PDF document or feel free to contact us.
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【Usage】 ■ Expression of target genes within cells ■ Functional analysis of proteins ■ Can be used for the production of useful proteins *For more details, please refer to the PDF materials or feel free to contact us.
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Yamaguchi TLO Co., Ltd. is a TLO approved by the Ministry of Education, Culture, Sports, Science and Technology and the Ministry of Economy, Trade and Industry based on the Act on the Promotion of Technology Transfer from Universities, established in November 1999 with investments from 50 faculty members of Yamaguchi University (capital of 4 million yen) before incorporation. It is a liaison-type technology transfer organization that bridges people. We return the research results from the university to society in the form of technology transfer and other means. Additionally, we are actively recruiting member companies to prioritize the disclosure of invention information and contribute to the development of the local economy through these efforts.