After thawing, it shows a high survival rate in various cultured cells and maintains the pluripotency of stem cells (undifferentiated state).
"Cryoscalis DMSO-Free" is a cell freezing preservation solution that allows for approximately 90% survival rate even after thawing, while maintaining the differentiation potential of stem cells during cryopreservation. It does not contain proteins or DMSO and can be stored stably at 4°C for long periods. Due to the absence of toxicity from DMSO and the influence of proteins, cells can be cryopreserved safely with a high survival rate. 【Features】 ■ Contains no serum or protein components ■ Approximately 90% survival rate even after thawing ■ Stem cells can be cryopreserved while maintaining their differentiation potential ■ Confirmed to be free from bacterial, fungal, and mycoplasma contamination through sterility testing ■ Can be stored stably at 4°C for long periods *For more details, please refer to the related links or feel free to contact us.
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【Overview of Operation Method】 ■ Place cultured cells in a tube, centrifuge, and remove the supernatant. ■ Add this product to suspend the cells to a concentration of 5×10^5 to 5×10^6 cells/ml, and aliquot 1 ml into storage tubes. ■ Freeze by placing in a -80℃ deep freezer. * For long-term storage, keep in liquid nitrogen. ■ Rapidly thaw the stored cells in a 37℃ incubator while shaking, and centrifuge wash with an appropriate medium (about 10 ml). * For more details, please refer to the related links or feel free to contact us.
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【Usage】 ■ Freezing preservation solution for cells *For more details, please refer to the related links or feel free to contact us.
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BMG is a research and development venture company that has been challenging the development of new biomaterial products adapted to trends in the medical field, based on a long-standing strong collaboration with the Institute for Frontier Medical Sciences at Kyoto University. Since its founding, the company has focused on the field of medical polymers to realize its corporate philosophy of "contributing to the health of people around the world by working on the development of better medical biomaterial products and providing innovative solutions in the medical field," supporting advancements in biomaterials technology and people's health across three business areas.