Ultrasonic uniform cell treatment (disruption and dissolution) for 96 well plates
Heelsher UIP400MTP 96-well plate dedicated ultrasonic homogenizer
The UIP400MTP is a device specially developed to perform ultrasonic treatment, such as cell disruption, uniformly using the 96WellPlete.
The Hielscher UIP400MTP is a dedicated ultrasonic processing device for 96-well plates. The purpose of cell and bacterial disruption is generally to extract specific components within the cells or bacteria (such as proteins and nucleic acids). Ultrasonic cell disruption has been a reliable method with established reproducibility over many years, but it has traditionally been difficult to uniformly process multiple sample specimens simultaneously. The UIP400MTP ultrasonic device can uniformly process 96 samples across various shapes of 96-well plates, achieving excellent cell disruption and extraction yields. Thanks to the custom-made sonotrode for wide areas, which is unique to Hielscher, uniform cavitation allows for uniform processing of 80-90% of the tubes in a 96-tube setup. Recovery rate = 80-90%.
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basic information
The German company Hielscher is a manufacturer of ultrasonic processors for dynamic applications. They have successfully minimized energy loss due to internal heating to nearly zero, allowing their lab devices to operate without a cooling mechanism, achieving compact design and an ultrasonic conversion efficiency of over 98%. They offer a lineup of industrial devices with a maximum output of 16,000W per unit. Both lab and industrial models guarantee continuous operation for 24 hours. The purpose of cell and bacterial disruption is generally to extract specific components from within the cells or bacteria (such as proteins and nucleic acids). Various methods are commonly used for cell disruption, including bead milling, pressure disruption (French press), and freeze-thaw cycles, but ultrasonic disruption is also widely used. Ultrasonic cell disruption using a bath is a reliable method with good reproducibility; however, it has been challenging to uniformly treat multiple sample specimens simultaneously. The ultrasonic device UIP400MTP can uniformly process 96 samples in various shapes of 96-well plates, achieving excellent cell disruption and extraction yields.
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P5
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Applications/Examples of results
In cell-based analyses such as ELISA and PCR, it is necessary to extract target molecules from within cells through cell lysis and cell disruption as a preprocessing step. Examples of applications: 1. Detachment of adherent cells 2. Dissolution of protein precipitates The purpose of cell or bacterial disruption is generally to extract specific components within the cells or bacteria (such as proteins and nucleic acids). Common methods for cell disruption include bead milling, pressure disruption (French press), and freeze-thaw cycles, but ultrasonic disruption is also widely used. Ultrasonic cell disruption using a bath is a reliable method with good reproducibility; however, it has been challenging to uniformly treat multiple samples simultaneously. The ultrasonic device UIP400MTP can uniformly process 96 samples in various shapes of 96-well plates, achieving excellent cell disruption and extraction yields.
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Company information
DKSH is a member of the Swiss global company "DKSH Group." It provides comprehensive services and solutions across the entire value chain, combining procurement, marketing, sales, distribution, and after-sales service, through its three business divisions: consumer goods, production materials, and technology, with a long-term perspective.