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  4. Cell culture
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  6. "Culture and purification of Escherichia coli" Recombinant Escherichia coli (E. coli)

"Culture and purification of Escherichia coli" Recombinant Escherichia coli (E. coli)

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last updated:Jul 05, 2022

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I want to acquire it quickly, in large quantities, and at a low cost! We accept protein expression under various conditions.

We would like to introduce the "E. coli Cultivation and Purification" handled by Unitec Co., Ltd. We can accommodate your desired cultivation volume, from a single Sakaguchi flask to fermenter cultivation. We propose suitable testing methods for those who wish to conduct large-scale cultivation and purification after considering the conditions. Our company has extensive experience in constructing expression vectors and protein expression. We accept protein expression under various conditions, including E. coli, yeast, insect cells, and animal cells. 【Cultivation and Protein Purification Using Recombinant E. coli】 - Optimization for codon usage (converting the nucleotide sequence to one that is easier to express in E. coli while keeping the amino acid sequence unchanged) - Exploration of induction conditions and cultivation conditions that facilitate more soluble expression - Refolding of insoluble fraction (inclusion body) expressed proteins is also possible - Estimated expression amount: 1–10 mg/L *For more details, please refer to the PDF materials or feel free to contact us.

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"Culture and purification of Escherichia coli" Recombinant Escherichia coli (E. coli)

2022-06-24_14h47_37.png
2022-06-24_14h47_37.png
  • Related Link - https://www.uniqtech.co.jp/protein-expression/

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【Project】 ■Construction of expression vector ■Examination of expression conditions (qualitative tests) ■Trial culture (250ml) ■Trial purification ■Option: Gel filtration column purification / Option: Ion exchange column purification ■Submission of interim report ■Main culture ■Main purification *For more details, please refer to the PDF materials or feel free to contact us.

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E. coli culture and purification

E. coli culture and purification

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Our company provides research support through services related to iPS cell contracts, genetically modified mice, animal experimentation contracts, cell-related contracts, protein expression purification and antibody production, and virus production. Please feel free to consult us if you have any requests.

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