Using episomal vectors! Achieving high-quality iPS cell production through advanced technology.
We would like to introduce "Establishment, Culture, and Differentiation Induction of iPS Cells" handled by Unitec Co., Ltd. Feeder-free culture uses an episomal vector for the production of human iPS cells. It is important to produce high-quality iPS cells, as this affects the quality of the cells after differentiation. The expression of undifferentiated markers is confirmed through flow cytometry analysis and immunofluorescence staining, and verification is also conducted through embryoid body formation. 【Features】 ■ Feeder-free culture ■ Use of episomal vector ■ Impact on the quality of cells after differentiation ■ Confirmation of undifferentiated marker expression ■ Verification through embryoid body formation *For more details, please refer to the PDF materials or feel free to contact us.
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【Real Time PCR Process】 ■Preparation of test plan ■Extraction and purification of total RNA ■cDNA synthesis ■Design and synthesis of primers ■Real-time PCR measurement ■Delivery *For more details, please refer to the PDF document or feel free to contact us.
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For more details, please refer to the PDF document or feel free to contact us.
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Our company provides research support through services related to iPS cell contracts, genetically modified mice, animal experimentation contracts, cell-related contracts, protein expression purification and antibody production, and virus production. Please feel free to consult us if you have any requests.