Explaining the internal standard method, external standard method, and considerations to keep in mind step by step!
The document includes the quantification of organic compounds and discusses internal and external standard methods. Quantitative NMR (qNMR) has become an efficient tool for quantifying organic compounds, and its use has been expanding in the pharmaceutical and chemical industries in recent years. Generally, proton NMR is used, but when considering the introduction of qNMR in new fields such as metabolomics, biomarker discovery, and physiological pathways, the presence of more complex molecules and systems makes the use of 1H-qNMR challenging. In such cases, using other NMR-active nuclei, namely 31P or 19F, may be a better option. [Contents (excerpt)] ■ Quantification of organic compounds ■ Internal and external standard methods ■ Considerations to take step by step *For more details, please refer to the PDF document or feel free to contact us.
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【Other Published Content】 ■Selection of appropriate standard materials ■Verification of compatibility ■Measurement and weighing ■Equipment setup ■Spectral evaluation ■Calculation of content ■Uncertainty *For more details, please refer to the PDF document or feel free to contact us.
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From research and development of pharmaceuticals to manufacturing and diagnostics, Merck is working to solve various challenges in life sciences in collaboration with the global science community. Merck's products include over 300,000 items, ranging from laboratory pure and ultra-pure water systems to gene editing tools for drug manufacturing, antibodies, cell lines, and end-to-end systems. To realize promising research more quickly and safely, we closely collaborate with customers in academia, the biotechnology industry, and the pharmaceutical industry. Please feel free to contact us for inquiries about our products or if you are interested in raw material supply. Here is the Merck ultra-pure and pure water systems webpage → https://www.merckmillipore.com/LW Here is the Merck life science reagents, raw materials, and analysis webpage → https://www.sigmaaldrich.com/JP/ja