Using the desalting tube "Sornak Tube" for LC/MS! Reduces protein ionization suppression.
We will introduce the analysis of intact proteins using "Sornack Tube" by MS Solutions. In the analysis of intact proteins using reversed-phase partition chromatography, a mobile phase containing trifluoroacetic acid (TFA) is used. However, TFA has a high acidity, which is known to increase the current flowing between the needle and the counter electrode when used in LC-ESI/MS, thereby suppressing the ionization of the analytes. For details on conditions and analysis results, please download the catalog. 【LC Conditions (Partial)】 ■ Equipment: Agilent 1200 ■ Flow rate: 0.3 ml/min ■ Detector: UV (210 nm) ■ Sample: Protein each at 10 pmol/μL solution ■ Injection volume: 5 μL *For more details, please refer to the PDF document or feel free to contact us.
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【MS Conditions (Partial)】 ■Device: JEOL JMS-T100LP ■Ionization Method: ESI Pos. ■Needle Voltage: 2000 V ■Orifice 1 Voltage: 100 V ■Desolvation Chamber Temperature: 250 ℃ *For more details, please refer to the PDF document or feel free to contact us.
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For more details, please refer to the PDF document or feel free to contact us.
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Our company develops contract services utilizing our unique high-level technical expertise in imaging mass spectrometry and liquid chromatography mass spectrometry. As a venture originating from Hamamatsu University School of Medicine, we leverage our unique technology related to IMS, cultivated over many years in the Cell Molecular Anatomy course, along with our extensive experience in mass spectrometry, including a Level 5 LC/MS analyst certification, to provide high-quality mass spectrometry data.