Using the desalting tube "Sornak Tube" for LC/MS! The effect of TFA removal allows for signal detection.
When analyzing acidic compounds using reversed-phase partition chromatography, an acidic mobile phase (set to a pH that is at least 2 lower than the pKa of the analyte) may be used to suppress dissociation. Common acidic mobile phases suitable for use in LC/MS include acetic acid, formic acid, and trifluoroacetic acid (TFA). While TFA is appropriate for use at low pH, it is believed to cause ionization suppression with nearly 100% probability when detecting the analyte as a negative ion. Using methionine, an amino acid with acidic groups and high polarity, as the sample, TFA was removed from the eluent using a Sorbent tube, and negative ion measurements were conducted. For details on conditions and analysis results, please download the catalog. 【LC Conditions (Partial)】 ■ Equipment: Agilent 1200 ■ Flow rate: 0.3 ml/min ■ Detector: UV (210 nm) ■ Sample: Methionine 20 ppm solution ■ Injection volume: 5 μL *For more details, please refer to the PDF document or feel free to contact us.
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【MS Conditions (Partial)】 ■Device: JEOL JMS-T100LP ■Ionization Method: ESI Neg. ■Needle Voltage: 2000 V ■Orifice 1 Voltage: 45 V ■Desolvation Chamber Temperature: 250 ℃ *For more details, please refer to the PDF document or feel free to contact us.
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For more details, please refer to the PDF document or feel free to contact us.
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Our company develops contract services utilizing our unique high-level technical expertise in imaging mass spectrometry and liquid chromatography mass spectrometry. As a venture originating from Hamamatsu University School of Medicine, we leverage our unique technology related to IMS, cultivated over many years in the Cell Molecular Anatomy course, along with our extensive experience in mass spectrometry, including a Level 5 LC/MS analyst certification, to provide high-quality mass spectrometry data.