[Application Data] Examples of improvements for easily adsorbed basic compounds

An example of improvement using post-column method for basic compounds that easily adsorb to the Sornak tube!

When using the Solnack tube CFAN under phosphate buffer conditions, if the compound being measured is basic, it may become dissociated and adsorb to the resin inside the tube. Therefore, by adding ammonia water using the post-column method before introducing the tube, we thought that making the eluent inside the tube basic and keeping the target compound in its non-dissociated form could suppress adsorption. We attempted to improve adsorption to the Solnack tube by adding ammonia water using the post-column method. For details on conditions and analysis results, please download the catalog. 【LC Conditions (Partial)】 ■ Equipment: Agilent 1200 ■ Detector: UV (215 nm) ■ Sample: 20 ppm solution of hydrochloride doxepin reagent ■ Injection volume: 5 μL ■ Post-column: 200 mM ammonia water at 0.05 ml/min *For more details, please refer to the PDF document or feel free to contact us.

Tubes and hoses