Application Data: Example of Nucleotide Measurement by TEA Removal

Using the desalting tube "Solnac Tube" for LC/MS! Reduces ionization suppression of nucleotides.

When analyzing highly polar nucleic acids and nucleotides using reversed-phase distribution chromatography, adding ionic species to the eluent can enhance retention by forming neutral ion pairs through ionic bonding. In the case of nucleic acid analysis, it is common to use triethylamine (TEA) as a basic mobile phase for ion pair formation; however, TEA is considered to cause ionization suppression when detecting positive ions due to its excessively high basicity. In this instance, adenylate (AMP) and other samples were used, and TEA in the eluent was removed using a solid-phase extraction tube for positive ion measurement. For details on conditions and analysis results, please download the catalog. 【LC Conditions (Partial)】 ■ Equipment: Waters UPLC H Class ■ Flow rate: 0.3 ml/min ■ Sample: Solutions of AMP, ADP, ATP at 50μM each ■ Injection volume: 10 μL *For more details, please refer to the PDF document or feel free to contact us.

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