Enzyme 'Bst DNA Polymerase' for isothermal gene amplification technology LAMP method
Bst DNA Polymerase
Bst DNA polymerase is an enzyme that can be used in the LAMP method. The LAMP method is a technique for amplifying and detecting target DNA or RNA in a short period of time.
This product is an enzyme that has 5'→3' DNA polymerase activity and strand displacement activity, capable of synthesizing new DNA strands while dissociating the hydrogen bonds of the double-stranded DNA template. The heat-stable strand-displacing DNA polymerase does not require the dissociation of double-stranded DNA due to its characteristics, allowing DNA synthesis at a constant temperature, and it is not affected by synthesis inhibition caused by the secondary structure of DNA. 【Features】 ■ Has 5'→3' DNA polymerase activity and strand displacement activity ■ Capable of DNA synthesis at a constant temperature ■ Suitable for synthesizing DNA strands with high GC content ■ Not inhibited by the secondary structure of DNA 【Optimal Reaction Temperature】 60°C to 65°C 【Inactivation Time】*1 80°C for 5 minutes *1 Inactivation temperature when the enzyme stock solution is directly heat-denatured 【Applications】 Applications utilizing strand displacement activity (such as isothermal gene amplification methods)
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basic information
【Product Contents】 ■ Capacity: 1,600 units ■ Components: Bst DNA Polymerase (8 units/μl) 200 μl x 1 10 × Bst Reaction Buffer (80 mmol/l Mg2+) 500 μl x 1 ■ Storage: -20°C
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P2
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Applications/Examples of results
【Usage】 ■ Applications utilizing chain replacement activity Example: LAMP (Loop-mediated isothermal amplification) method, etc. *For more details, please refer to the related links or feel free to contact us.
Detailed information
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【Experimental Example】 ■Confirmation of LAMP Reaction by Electrophoresis Reaction Conditions Volume: 25.0 µl Template DNA: Citrus Greening Disease Diagnostic Kit HLB Positive Control (1x 10^6 copies/reaction) Reaction Temperature: 65˚C Reaction Time: 60 minutes Enzymes Used: Bst DNA Polymerase (Company N) 8 units Bst DNA Polymerase (Nippon Gene: NPG) 8 units
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Company information
Our company is a product development-oriented enterprise that utilizes biotechnology. We develop and manufacture in vitro diagnostic pharmaceuticals (such as pregnancy tests and ovulation tests), diagnostic drugs for environmental, food, and animal and plant diseases, and reagents for genetic engineering research, using our expertise in gene and antibody technology. We hold manufacturing registration and manufacturing and sales licenses for both human and animal products, with over 30 years of experience in the manufacture of in vitro diagnostic pharmaceuticals. Additionally, we are engaged in personalized medicine, advancing research and development of diagnostic drugs using genetic polymorphism analysis and gene expression analysis.