Three-dimensional cell culture substrate "HYDROX"
66025/66026 Three-Dimensional Nanofiber "HYDROX" Powder Regular/Large
The new type of three-dimensional cell culture substrate "HYDROX" allows for three-dimensional culture of various types of cells simply by seeding a cell suspension.
Features of HYDROX - Derived from chemically synthesized polymers - Ready-to-use, can be stored at room temperature - Forms spheroids simply by seeding cells - Easy recovery of cells after culture - Confirmed usefulness data for culturing liver and nerve cells Currently, in three-dimensional culture, animal-derived components are primarily used as scaffolding materials to promote interactions between cells and between cells and the extracellular matrix. However, the use of animal-derived components has raised issues regarding reproducibility, safety, and handling difficulties. Shimadzu Corporation's three-dimensional nanofiber "HYDROX" is a new type of three-dimensional cell culture substrate using polysarcosine-polylactic acid polymer. It enables three-dimensional culture of various types of cells simply by seeding a cell suspension.
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basic information
HYDROX is a powdered reagent made from amphiphilic polymers consisting of polylactic acid and polysarcosine, without using any animal-derived components. The HYDROX polymer powder dissolved in ethanol is dropped onto a culture plate and dried to form a dry gel on the bottom surface of the plate. 1) Add a cell suspension to the created gel. 2) The dried gel absorbs moisture and forms three-dimensional nanofibers called "HYDROX." 3) During this process, the cells trapped in the nanofibers aggregate, forming a three-dimensional cell mass. 4) By adding an excess amount of moisture, the structure of the HYDROX nanofibers is disrupted, allowing for easy recovery of the cell mass alone.
Price information
66025 Three-Dimensional Nanofiber "HYDROX" Powder Regular 30 mg 25,000 yen 66026 Three-Dimensional Nanofiber "HYDROX" Powder Large 140 mg 75,000 yen
Price range
P2
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Applications/Examples of results
Immature liver cells induced to differentiate for 14 days from human iPS cells were cultured three-dimensionally using HYDROX. The immature liver cells cultured with HYDROX aggregated over time to form cell clusters (spheroids). Development of Polysarcosine-Poly-Lactic Acid (PSar-PLLA) Polymer Gel, Shimadzu Review, Vol. 75[3・4], 113–121 (March 2019) Development of a Novel Three-Dimensional Culture Substrate HYDROX: Evaluation of Differentiation Induction from Human iPS Cells to Liver Cells and Application to Primary Culture of Human Liver Cells, Shimadzu Review, 78[3・4], 193–202 (2021)
Detailed information
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HYDROX is a new product developed by Shimadzu Corporation and is currently in test sales. For inquiries regarding reagent performance and sales, please contact Shimadzu Diagnostics. We also accept technical consultations.
Line up（2）
| Model number | overview |
|---|---|
| 66025 | Three-dimensional nanofiber "HYDROX" powder regular 30 mg |
| 66026 | Three-dimensional nanofiber "HYDROX" powder large 140 mg |
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Company information
"Creating new businesses through open innovation, providing services and standards globally online." Shimadzu Diagnostics Co., Ltd. is a company engaged in the development of a wide range of products, including diagnostic reagents that meet diverse needs in clinical testing and industrial reagents that support hygiene management in the food industry. We are committed to providing high-quality products and services more quickly to meet our customers' expectations.