High-sensitivity immunoassay using nanoantibody-presenting polysaccharides [Saitama University]
By combining nanoantibodies with polysaccharides to create "nanoantibody-presenting polysaccharides," it is possible to immobilize nanoantibodies onto substrates.
○Overview of the Technology We have developed a method to improve detection sensitivity using enzyme-linked immunosorbent assay (ELISA) with nanobodies. We prepared a nanobody-polysaccharide conjugate by chemically linking nanobodies to water-soluble polysaccharides, and it was confirmed that the sensitivity in the solid-phase ELISA using this conjugate was improved compared to using the nanobodies alone. ○Comparison with Conventional Technology In ELISA using nanobodies as a substitute for conventional antibodies, there was a challenge of inactivation due to solid-phase immobilization and detachment from the substrate. This technology allows for the improvement of ELISA detection sensitivity by using water-soluble polysaccharides as a scaffold for solid-phase immobilization of nanobodies. ○Features of the Technology - Application of nanobodies in sandwich ELISA - Water-soluble polysaccharides protect nanobodies - Improved binding affinity due to aggregation of nanobodies - Suppression of nonspecific adsorption
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This technology is a research outcome from Saitama University. We hope to collaborate with companies for practical application through joint research and development. If you have any requests for product development in collaboration with university technology, please feel free to contact us.
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(Expected Applications) - Test reagents and diagnostic drugs using nanobodies - Stabilization of nanobodies - Surface coating agents for presenting nanobodies on substrates (Patent Information) Japanese Patent Application No. 2017-008331 Antibody-polysaccharide conjugates and highly sensitive immunoassay methods using them
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