Tohoku University Technology:Target specific and non-biased gene amplification: T14-107
TGene expression analysis is possible without changing copy number ratio in a sample!
In comprehensive gene expression analysis such as T cell receptor (TCR) repertoire analysis, it is important that target specific and non-biased gene amplification products are obtained. Conventional methods often ligate adapters with restriction enzyme site to synthesized cDNA ends and treat adapter-ligated cDNAs with said restriction enzyme before specific amplification. In such methods, however, unused and untreated adapters often inhibit target specific and non-biased amplification. This invention performs target specific, non-biased gene amplification by combination of uracil-containing adapter and uracil DNA glycosylase (UNG) treatment (Fig 1).
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The revenue generated from technology transfer is reinvested as new research funding for universities and researchers, and is utilized to create further research outcomes. To ensure the smooth operation of this cycle, known as the "Intellectual Creation Cycle," we will vigorously promote technology transfer. The types of seeds we handle include patents, know-how, databases, and programs. We have established a collaborative framework by signing basic technology transfer agreements with the following universities (as of June 1, 2025): Tohoku University, Hirosaki University, Iwate University, Akita University, Fukushima University, Yamagata University, Tohoku Gakuin University, Iwate Medical University, Fukushima Medical University, Aizu University, Miyagi University, Hokkaido University, Muroran Institute of Technology, and Showa Medical University.
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