Introducing a scalable AAV purification process and a method for separating full capsids and empty capsids using anion exchange chromatography.
Adeno-associated virus (AAV) is widely used as a vector for gene therapy. While it is often purified using ultracentrifugation at the lab scale, this method becomes difficult to scale up. A scalable method for purification is chromatography; however, separating full capsids (hereafter referred to as "full") that contain the genome from empty capsids (hereafter referred to as "empty") that do not contain the genome and do not contribute to titer has been challenging. When using AAV as a vector for gene therapy, it is desirable to remove empty capsids as much as possible due to their impact on titer and issues related to immunogenicity. This application note introduces a scalable AAV purification process and presents a method for separating empty and full capsids using anion exchange chromatography across multiple serotypes.
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Cytiva and Pall Life Sciences have merged their businesses and will officially launch under the "Cytiva" brand on May 1, 2023. Under our mission of "Advancing therapies, faster," we will integrate both organizations and focus even more on supporting the development and manufacturing of better therapies for patients as a stronger global provider of technologies and services. The new Cytiva will offer a broader and deeper expertise and a rich lineup of products and services to better support various customers involved in research and the manufacturing and development of biopharmaceuticals, enabling them to deliver therapies to patients more quickly, from discovery to delivery. With a rich history spanning several hundred years, Pall Life Sciences and Cytiva, as part of the Danaher Group, will continue to unite to provide better services to our customers.