Cytiva Protein Select for Recombinant Protein Purification

Self-disconnecting tags - high-purity refinement in one step.

Cytiva Protein Select resin allows for the spontaneous cleavage between the tag and the target protein during the affinity step, resulting in the elution of high-purity target protein without any residual amino acids from the tag. 【Self-Cleavage of the Tag】 The Cytiva Protein Select tag binds to the ligand on the resin, and after a certain period, the tag is cleaved from the target protein. This means that no enzymatic digestion is required! 【No Residual Amino Acids from the Tag】 Since the cleavage occurs between the tag and the target, no amino acids from the tag remain in the target protein. 【High Purity in One Step】 The cleaved target can be eluted from the column simply by flowing the starting buffer. There is no need for pH changes or the addition of reagents for elution.

Chromatography