Protein purification at the microscale - Ideal for small-scale multi-sample purification of antibodies, proteins, AAV, plasmids, and more.
PhyTip is a unique micro-scale chromatography that employs the patented dual-flow chromatography (DFC) technology, which is a foundational technology. It enables micro-scale purification of antibodies, AAV, proteins, and plasmids. The resin can be used from as little as 5 µl, allowing for small-scale protein purification, concentration, and high-throughput applications. Due to its chip-type chromatography, it is compatible with major liquid handlers. The use of PhyTip columns requires low-speed pipetting operations, necessitating specialized electric pipettors like the 'ME System' or liquid handler robots. It supports a wide range of resins and custom packing options. Key PhyTip column products include: ▪ Antibody purification: CaptureSelect PhyTip ProPlus LX (MabSelect SuRe) Columns, etc. ▪ AAV purification: CaptureSelect AAVX ▪ Affinity protein purification: IMAC PhyTip Columns, etc. ▪ Gel filtration ▪ Ion exchange ▪ Plasmid purification (mini prep)
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basic information
Resin amount: Minimum 5ul to maximum 360ul Chip sizes used: 200ul, 300ul, 500ul, 1000ul QTY/Box: 96 *For more details, please visit our website or feel free to contact us.*
Price range
P3
Delivery Time
P4
Applications/Examples of results
Antibody purification with a small number of samples, as well as the purification of recombinant proteins and AAV, is optimal for screening. It is compatible with liquid handlers from major companies such as Beckman Coulter and TECAN, and over 5,000 types of tips are available. Antibody Purification ProPlus PhyTip CaptureSelect PhyTip / ProPlus LX (MabSelect SuRe) CaptureSelect FcXLc CaptureSelect IgG-Fc (ms) CaptureSelect KappaXL CaptureSelect LC-Lambda Hu CaptureSelect IgM AAV Purification CaptureSelect AAVX Affinity Protein Purification IMAC PhyTip Streptavidin PhyTip Glutathione PhyTip Gel Filtration 5K Gel Filtration PhyTip 30K Gel Filtration PhyTip Ion Exchange Reverse Phase Normal Phase Miniprep, etc.
Detailed information
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Dual-flow chromatography technology A hydrophilic screen is fixed above and below the resin filled in the chip, and unlike conventional unidirectional chromatography, DFC allows for repeated suction and discharge, improving the binding capacity with the resin. With the realization of pipetting operations, reproducible data can be obtained using electric pipettors or liquid handlers. Additionally, pipetting at low speeds enables purification under mild conditions without losing the original activity of the proteins.
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Comparison of DFC and column purification, magnetic beads DFC is scalable, highly binding, can be concentrated, and can be used with small volumes of liquid.
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Company information
Biotage Japan Co., Ltd. is a wholly-owned subsidiary of Biotage AB, which is headquartered in Uppsala, Sweden (BIOT: NASDAQ/OMX Stockholm). Biotage AB has its roots in several venture companies that originated from Uppsala University, a leading science and engineering university in the Nordic region. After undergoing international acquisitions and restructuring throughout the 2000s, it has recently achieved remarkable growth as a global company.
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