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  5. Separation device
  6. Pipette tip-type small volume ion exchange PhyTip column

Pipette tip-type small volume ion exchange PhyTip column

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last updated:Aug 30, 2024

バイオタージ・ジャパン
バイオタージ・ジャパン
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Ideal for ion exchange on a microscale! Ion exchange PhyTip columns compatible with HTS.

Ion exchange chromatography is used as an orthogonal chemistry to obtain high-purity final products, serving as a second polishing step after another purification process or affinity chromatography purification. Ion exchange PhyTip columns are chip-type columns that utilize Dual Flow Chromatography technology, making them suitable for efficient parallel purification of small amounts of protein samples. They are compatible with major liquid handlers for chip-type chromatography. The use of PhyTip columns requires pipetting operations at low speeds, necessitating dedicated electric pipettors like the 'ME System' or liquid handler robots. They can accommodate a wide variety of resins and custom packing is also available. Product Information (4 products): Strong Anion exchange Weak Anion exchange Strong Cation exchange Weak Cation exchange

    Separation device
ion_exchange_vol.jpeg

Pipette tip-type small volume ion exchange PhyTip column

ion_exchange_vol.jpeg
ion_exchange_vol.jpeg
  • Related Link - https://www.biotage.co.jp/products_top/biomolecule…

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basic information

Resin volume: minimum 5ul to maximum 360ul Chip sizes used: 200ul, 300ul, 500ul, 1000ul QTY/Box: 96

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Applications/Examples of results

It is an ion exchange PhyTip suitable for sample processing on a microscale and compatible with HTS using 96-well plates.

Detailed information

  • スクリーンショット

    Dual Flow Chromatography Technology Hydrophilic screens are fixed above and below the resin filled in the chip, allowing for repeated suction and discharge, which improves the binding capacity with the resin, unlike conventional unidirectional chromatography. The realization of pipetting operations enables reproducible data to be obtained through the use of electric pipettors and liquid handlers. Additionally, pipetting at low speeds allows for purification under mild conditions without losing the original activity of the proteins.

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Purification of biomolecules using the PhyTip column

Purification of biomolecules using the PhyTip column

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Ion exchange PhyTip column

Ion exchange PhyTip column

TECHNICAL
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Company information

バイオタージ・ジャパン

バイオタージ・ジャパン

Testing, Analysis and Measurement

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Biotage Japan Co., Ltd. is a wholly-owned subsidiary of Biotage AB, which is headquartered in Uppsala, Sweden (BIOT: NASDAQ/OMX Stockholm). Biotage AB has its roots in several venture companies that originated from Uppsala University, a leading science and engineering university in the Nordic region. After undergoing international acquisitions and restructuring throughout the 2000s, it has recently achieved remarkable growth as a global company.

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