Comprehensive HTS from His tag purification in small amounts! IMAC PhyTip column
The IMAC PhyTip column allows for easy affinity purification in small amounts, starting from a minimum resin volume of 5 µL. If you want to process recombinant protein purification in small quantities with multiple samples, it can be handled using HTS. It is compatible with major liquid handlers for chip-type chromatography. The use of the PhyTip column requires slow pipetting operations, so a dedicated electric pipetter, such as the 'ME System', or a liquid handler robot is necessary. It supports a wide variety of resins and custom packing is also available.
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basic information
Resin amount: Minimum 5ul to maximum 360ul Chip sizes used: 200ul, 300ul, 500ul, 1000ul QTY/Box: 96
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Applications/Examples of results
▪Micro-scale purification of His-tag fusion proteins ▪HTS
Detailed information
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Dual Flow Chromatography Technology Hydrophilic screens are fixed above and below the resin filled in the chip, and unlike conventional unidirectional chromatography, DFC allows for repeated suction and discharge, improving the binding capacity with the resin. The realization of pipetting operations enables reproducible data to be obtained through the use of electric pipettors and liquid handlers. Additionally, pipetting at low speeds allows for purification under mild conditions without losing the original activity of the proteins.
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Binding capacity of IMAC PhyTip column
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Company information
Biotage Japan Co., Ltd. is a wholly-owned subsidiary of Biotage AB, which is headquartered in Uppsala, Sweden (BIOT: NASDAQ/OMX Stockholm). Biotage AB has its roots in several venture companies that originated from Uppsala University, a leading science and engineering university in the Nordic region. After undergoing international acquisitions and restructuring throughout the 2000s, it has recently achieved remarkable growth as a global company.