Maintenance of undifferentiated state in iPS cells

Evaluation of the undifferentiated maintenance ability of a dimeric peptide composed of cyclic peptides that bind to human FGFR1c.

We present the results of evaluating the undifferentiated maintenance ability of FGF2 and the FGF2 alternative peptide "PG-011" using three types of human iPS cell lines. FGF2 (100 ng/mL, approximately 5.9 nM) or an equimolar concentration of PG-011 (approximately 30 ng/mL) was added to the culture medium, and after 7 days of culture, passaging was performed. Three passages were conducted, and the positive rate of undifferentiated markers in iPS cells on Day 7 of passage 3 (P3) was evaluated. As a result, the cell numbers at each passage were almost the same, confirming that "PG-011" exhibited similar proliferation ability to FGF2 in iPS cells. [Results] - iPS cells cultured with "PG-011" and FGF2 showed similar colony morphology. - The cell numbers at each passage were almost the same, confirming that "PG-011" exhibited similar proliferation ability to FGF2 in iPS cells. - The measurement results of undifferentiated markers in iPS cells on P3, Day 7 indicated that "PG-011" has an undifferentiated maintenance ability at the same level as FGF2. *For more detailed test results, please download the materials from the link below.

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